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Nucleotide Triplet

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Nucleotide Triplet
NA QUESTION 1982: L. PETERSON/AP BIOLOGY

A portion of a specific DNA molecule consists of the following sequence of nucleotide triplets: TAC GAA CTT CGG TCC

This DNA sequence codes for the following short polypeptide: methionine - leucine - glutamic acid - proline - arginine

Describe the steps in the synthesis of this polypeptide. What would be the effect of a deleltion or an addition in one of the DNA nucleotides? What would be the effect of a substitution in one of the nucleotides?

STANDARDS: In the transcription phase of protein synthesis, students were given a point for a correct definition of transcription. They were awarded one point for mentioning each of the following: …show more content…
__ DNA as the template molecule for messenger RNA __ The proper base pairing (including the uracil substitution) __ The chemical characteristics of nucleotides __ A comparison of RNA and DNA (other than uracil substitution) __ The triplet arrangement of codons and/or anticodons __ The control of transcription (Operon, etc.) __ Promoters __ The role of polymerase __ Intervening sequences in eukaryotic cells __ Factors involved in the release of mRNA from DNA __ 5' - 3' arrangement with attachment at -OH end A definition of translation was worth an additional point with one point given for mention of each of the following: __ Movement of mRNA from nucleus to ribosome __ Association of mRNA with the ribosome and ribosomal RNA __ Location of ribosomes __ Description of ribosome/polyribosome structure __ Role of transfer RNA in transporting amino acids __ Specific characteristics of tRNA __ Amino acid-tRNA complex activation __ Role of ATP and enzymes __ tRNA initiator molecules (met or f-met) __ Initiation factors (GTP, etc.) resulting in union of 30s and 50s __ Bonding of tRNA to 50s (A
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