Odontogenic Tumors Lab Report
MATRIX METALLOPROTEINASE EXPRESSION IN ODONTOGENIC TUMOR CELL POPULATIONS
Monée Casimir, Dr. Jesse Mason
Introduction:
Ameloblastomas (AB) and calcifying epithelial odontogenic tumors (CEOT) are rare tumors thought to develop from the odontogenic epithelium and invade the jaw bone and local tissue. Keratocystic calcifying odontogenic tumors (KCOT) are also rare odontogenic tumors which are locally invasive, but do not invade the bone. The purpose is to compare expression of matrix metalloproteinases (MMPs) between odontogenic tumors that invade the jaw bone and tumors that do not. MMPs are enzymes that degrade extracellular matrix proteins and play a role in normal and tumor cell migration. Certain MMPs have been shown previously to play a role in ameloblastoma bone invasion. MMP-1, MMP-2, MMP-11, MMP-16, MMP-17, MMP-21, and MMP-24 were shown to play role in odontogenic tumor invasions. …show more content…
Objectives:
To determine which MMPs are involved in odontogenic tumor invasion.
Materials and Methods:
Previously developed primary cell populations from four ameloblastomas, four KCOTs, and one CEOT were grown in DMEM supplemented with 10% FBS and antibiotics. One sample for each tumor subtype (AB-1, KCOT-1, and CEOT-1) was analyzed by cDNA microarray. Cells were collected and analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry to determine MMP expression.
Results and Discussion:
MMPs were generally expressed broadly amongst tumor samples. MMP-1, MMP-3, MMP12, and MMP-16 were differentially expressed in tumor cell populations by microarray analysis. MMP-12 expression was increased in the KCOT tumor subtype compared to AB and CEOT tumors similar to microarray data. Differences in the expression of certain MMPs could be seen between tumor types.
Conclusion:
Distinct expression of MMPs may be involved in the differences in bone invasion observed amongst odontogenic tumors.
Future Directions:
Repeat qRT-PCR experiments for identified MMPs to provide statistical analysis.
Perform immunohistochemical analysis of MMPs identified by qRT-PCR.
Perform western blot analysis on MMPs that were differentially expressed amongst tumor subtypes.
References:
Hegedus, L, Cho H, Xie, X, Eliceiri. 2008. J Cell Physiol. 216: 480-5.
Khalifa GA, Shokier HM, Abo-Hager EA. 2010. J Egypt Natl Canc Inst. 22: 61-72.
Mandal S, Varma K, Khurana N, Mandal AK. 2008. Indian J Pathol Microbiol. 51:397-8.
Qian Y, Huang HZ. 2010. J Oral Pathol Med. 39: 592-8.
Ren C, Diniz MG, Piazza C, Amm HM, Rollins DL, Rivera H, Devilliers P, Kestler DP, Waite PD, Mamaeva OA, Macdougall M. 2011. Cell, Tissue, Organs. Epub May 19.
Sharif, FN, Oliver R, Sweet C, Sharif MO. 2010. Cochrane Database Syst Rev. 9: CD008464.
Zhang B, Zhang J, Xu ZY, Xie HL. 2009. BMC Cancer. 9: 427-32.
Monée Casimir, Dr. Jesse Mason
Introduction:
Ameloblastomas (AB) and calcifying epithelial odontogenic tumors (CEOT) are rare tumors thought to develop from the odontogenic epithelium and invade the jaw bone and local tissue. Keratocystic calcifying odontogenic tumors (KCOT) are also rare odontogenic tumors which are locally invasive, but do not invade the bone. The purpose is to compare expression of matrix metalloproteinases (MMPs) between odontogenic tumors that invade the jaw bone and tumors that do not. MMPs are enzymes that degrade extracellular matrix proteins and play a role in normal and tumor cell migration. Certain MMPs have been shown previously to play a role in ameloblastoma bone invasion. MMP-1, MMP-2, MMP-11, MMP-16, MMP-17, MMP-21, and MMP-24 were shown to play role in odontogenic tumor invasions. …show more content…
Objectives:
To determine which MMPs are involved in odontogenic tumor invasion.
Materials and Methods:
Previously developed primary cell populations from four ameloblastomas, four KCOTs, and one CEOT were grown in DMEM supplemented with 10% FBS and antibiotics. One sample for each tumor subtype (AB-1, KCOT-1, and CEOT-1) was analyzed by cDNA microarray. Cells were collected and analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry to determine MMP expression.
Results and Discussion:
MMPs were generally expressed broadly amongst tumor samples. MMP-1, MMP-3, MMP12, and MMP-16 were differentially expressed in tumor cell populations by microarray analysis. MMP-12 expression was increased in the KCOT tumor subtype compared to AB and CEOT tumors similar to microarray data. Differences in the expression of certain MMPs could be seen between tumor types.
Conclusion:
Distinct expression of MMPs may be involved in the differences in bone invasion observed amongst odontogenic tumors.
Future Directions:
Repeat qRT-PCR experiments for identified MMPs to provide statistical analysis.
Perform immunohistochemical analysis of MMPs identified by qRT-PCR.
Perform western blot analysis on MMPs that were differentially expressed amongst tumor subtypes.
References:
Hegedus, L, Cho H, Xie, X, Eliceiri. 2008. J Cell Physiol. 216: 480-5.
Khalifa GA, Shokier HM, Abo-Hager EA. 2010. J Egypt Natl Canc Inst. 22: 61-72.
Mandal S, Varma K, Khurana N, Mandal AK. 2008. Indian J Pathol Microbiol. 51:397-8.
Qian Y, Huang HZ. 2010. J Oral Pathol Med. 39: 592-8.
Ren C, Diniz MG, Piazza C, Amm HM, Rollins DL, Rivera H, Devilliers P, Kestler DP, Waite PD, Mamaeva OA, Macdougall M. 2011. Cell, Tissue, Organs. Epub May 19.
Sharif, FN, Oliver R, Sweet C, Sharif MO. 2010. Cochrane Database Syst Rev. 9: CD008464.
Zhang B, Zhang J, Xu ZY, Xie HL. 2009. BMC Cancer. 9: 427-32.