enzymes within the chloroplasts; this would explain why the transmittance actually decreased in the body temperature tube. The hypothesis was rejected because of the fact that photosynthesis did not continuously increase with temperature as shown by the body temperature tube.
The room temperature tube not placed in a bath acted as the control due to the fact that it represented normal conditions with no constant bath temperature around. An interesting observation made from Figure 1 and Table 2 was that the room temperature tubes hade relatively different rates of photosynthesis even though they were at the same temperature. This could show that temperature is not the only determining factor is the rate of photosynthesis. An explanation for an the room temperature tube in the bath having a rate of 1.86 %T/min as opposed to the .75%T/min of the room temperature tube without a bath is that there may have been more water in the air around the bath, so the chloroplasts were able to absorb more water while in the tube to speed up the rate. The chloroplasts within the tube not in a bath may not have had access to as much water in the air, thus causing a lower rate of photosynthesis. Table 2 also shows that a larger difference in transmittance from 0 minutes to 15 minutes, yielded a larger rate of photosynthesis. A faster rate of photosynthesis also forced transmittance to increase as the solution became
clearer. This is because as the chloroplasts are going through photosynthesis, they are absorbing the dye, DPIP, in the solution. As the rate increases, more DPIP is absorbed which leads to a higher transmittance because with less dye, the solution becomes more clear. The rate of photosynthesis is important because it can provide insight into the optimal environment for a plant to begin photosynthesizing as efficiently as possible. More efficient photosynthesis means more oxygen given if by plants; this is beneficial to most organisms. There were sources of error in this experiment that could have potentially affected the results. After the chloroplasts were placed into the tubes and the tubes were inverted, each tube was inverted by a different person. This led to a different amount of time the solution was being inverted; this could potentially have led to some solutions being mixed more than others. This would be an example of systematic error as all tubes could have been inverted for the same length of time. A random source of error could be the amount of moisture in the atmosphere. More moisture could potentially affect the rate of photosynthesis slightly.