Pseudomonas Cepacia Lab Report
Biosynthesis, characterization and applications of biosurfactant produced by Pseudomonas cepacia using de-oiled rice-bran
Garima Bhardwaj1, Swaranjit Singh Cameotra2, Harish Kumar Chopra1*
1*Department of Chemistry, Sant Longowal Institute of Engineering and Technology, Longowal - 148106,
Distt. Sangrur (Pb.), India. E-mail: hk67@rediffmail.com
Fax: 91-1672-280072; Tel: 91-1672-305204 (O)
2Institute of Microbial Technology, Sector-39-A, Chandigarh-160036, India
Abstract: Pseudomonas cepacia MTCC 7099 was studied for the biosurfactant production using de-oiled rice-bran, which is a waste product of rice-bran oil industry. When Pseudomonas cepacia was grown in fermentation media containing de-oiled rice-bran, the reduction in surface tension observed was 33.2 mN/m at temperature 30 °C, pH 6.7±0.2, and shaking speed150 rpm after 96 h of incubation. The cell free supernatant was extracted many times with various …show more content…
is known for the production of rhamnolipid biosurfactants (Koch et. al., 1991; Jeong et. al., 2004, Costa et. al., 2004). Pseudomonas cepacia has also been known for the production of glycolipid biosurfactant (Silva et. al., 2014). Biosurfactant have recently received considerable attention in the field of environmental remediation processes due to their efficacy as dispersion and remediation reagents as well as their environmentally friendly characteristics such as low toxicity and high biodegradability. All these applications have led to the synthesis of biosurfactants by using even synthetic procedures with greener approach using inulin which is the second most abundant plant storage polysaccharide (Kokubun et. al., 2013). Besides of all these applications the only problem in the industrialization of biosurfactants is their relatively higher production costs compared to synthetic surfactants. The only way out to overcome this challenge is the use of various alternative, inexpensive substrates, such as agro-industrial
Garima Bhardwaj1, Swaranjit Singh Cameotra2, Harish Kumar Chopra1*
1*Department of Chemistry, Sant Longowal Institute of Engineering and Technology, Longowal - 148106,
Distt. Sangrur (Pb.), India. E-mail: hk67@rediffmail.com
Fax: 91-1672-280072; Tel: 91-1672-305204 (O)
2Institute of Microbial Technology, Sector-39-A, Chandigarh-160036, India
Abstract: Pseudomonas cepacia MTCC 7099 was studied for the biosurfactant production using de-oiled rice-bran, which is a waste product of rice-bran oil industry. When Pseudomonas cepacia was grown in fermentation media containing de-oiled rice-bran, the reduction in surface tension observed was 33.2 mN/m at temperature 30 °C, pH 6.7±0.2, and shaking speed150 rpm after 96 h of incubation. The cell free supernatant was extracted many times with various …show more content…
is known for the production of rhamnolipid biosurfactants (Koch et. al., 1991; Jeong et. al., 2004, Costa et. al., 2004). Pseudomonas cepacia has also been known for the production of glycolipid biosurfactant (Silva et. al., 2014). Biosurfactant have recently received considerable attention in the field of environmental remediation processes due to their efficacy as dispersion and remediation reagents as well as their environmentally friendly characteristics such as low toxicity and high biodegradability. All these applications have led to the synthesis of biosurfactants by using even synthetic procedures with greener approach using inulin which is the second most abundant plant storage polysaccharide (Kokubun et. al., 2013). Besides of all these applications the only problem in the industrialization of biosurfactants is their relatively higher production costs compared to synthetic surfactants. The only way out to overcome this challenge is the use of various alternative, inexpensive substrates, such as agro-industrial