Very often in the synthesis of a compound or in the isolation of a compound from a natural source, acid-base extractions are used to either directly isolate a desired acidic or basic compound or to remove acidic or basic impurities. Extraction, along with recrystallization and distillation, is one of the most important separation and purification techniques in organic chemistry.
The Experiment.
then the drop will mix with the solution. If the solvent is the mistaken organic layer, then the water drop will create a second layer. In general, this method can help determine the identity of the layer.
However, it is still best to keep ALL the layers until the extraction is complete and your product has been isolated.
Separating the Layers
Once the two layers are mixed, you will need to separate them. You could separate the two layers by pouring off the less dense layer into a separate container. You would find that it is difficult to do this