Preview

Qualitative Food Tests

Better Essays
Open Document
Open Document
1577 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Qualitative Food Tests
Name: Marcus Gonzales
Date: Wednesday 12th September 2012
Form: L62
Subject: Biology
Teacher: Ms. Hutton
Lab #: 1
Title: Food Tests
Aim: To test an unknown substance for various food substances.
Apparatus/Materials:
* Unknown Solution X * NaOH * Iodine * Ethanol * Copper Sulphate * Benedict’s Solution * HCl * Cold Water * Syringe * Beakers * Bunsen burner * Tripod stand * Test tubes * Dropper
Introduction: This Lab shows the detection of different food sources using different tests. Some of the food tests carried out was Benedict’s test, the test for non-reducing sugars, iodine test (test for starches), emulsion test and the biuret test.
Carbohydrates include reducing and non-reducing sugars also starches. They have a general Formula of CnH2nOn.They can be classified into three main groups, Monosaccharide, Disaccharide and polysaccharide. Carbohydrates have many uses such as: for energy, for storage of food in both plant and animal cells e.g. Starch, chloroplasts, for structure in plant cells e.g. cellulose have a structural use in plant cell walls giving it structure. Carbohydrates are sweet and soluble in water with the only exceptions of starch and cellulose. Reducing sugars react with the copper in Benedict’s reagent to form a brick-red precipitate. Non-reducing sugars however do not react with Benedict’s reagent because the part that would react with the Benedict’s reagent is already in a glycosidic bond with another molecule. Therefore it is heated with HCl to break these bonds so that the benedict’s solution can be effective. Starch is made up of amylose and amylopectin, it is used as storage for food made in plants.
Lipids can be classified as fats, oils, or anything similar to a fat/oil that is insoluble in water. There are different types of Lipids such as: Saturated, unsaturated, triglycerides and phospholipids Lipids are non-polar and insoluble in water. They are made

You May Also Find These Documents Helpful

  • Powerful Essays

    Ap Biology Unit 1 Summary

    • 2660 Words
    • 11 Pages

    * Used by organisms as sources of energy, as building materials, and as cell surface markers for cell-to-cell identification and communication…

    • 2660 Words
    • 11 Pages
    Powerful Essays
  • Good Essays

    Biochemistry GRT1   Lipids are molecules that are not soluble in water but can be dissolved in non-polar substances such as alcohol or chloroform (Mandal, n.d.). Lipid molecules are stored in adipose, or fat tissue, and can be broken down in our bodies to form an energy called ATP. To do so, the lipid molecule is dissected into its base pieces, glycerol and fatty acids. Each of the fatty acids is then broken down into two-carbon pieces and acetyl CoA is formed from each carbon piece.…

    • 476 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Lipids-One of a diverse group of organic substances all of which are relatively insoluble in water, but soluble in alcohol, ether, chloroform, or other fat solvents. are organic compounds of fatty acids, which are insoluble in water and soluble in organic solvents.…

    • 1050 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Fats - Fats are a complex of fatty acid chains attached to a glycerol backbone. Fats are a type of lipid, and are used for energy, energy storage, structure, hormones, waterproofing, and insulation. Types of food would depend on the fats, saturated or unsaturated. Butter, oils, certain meats, and certain foods all contain a certain amount of lipid source.…

    • 702 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Lipids (also known as fats) are nonpolar, insoluble molecules gained within the body trough digestion of food. Triglycerides, a type of lipid that serve as storage units for energy have 3 fatty acid chain tails made up of carboxylic acid and a fatty carbon chain attached to a glycerol backbone. These molecules can be saturated or unsaturated contingent on the bond types and hydrogen number in the molecule. Anytime a fatty acid is removed from a glyceride molecule, dehydration synthesis occurs and a water molecule is taken away. This helps to break the bonds, also called esterase linkages, within the molecule. These bonds house the energy within the triglyceride. After…

    • 740 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    The point of this lab is to determine if a substance contains carbohydrates such as a reducing sugar and/or polysaccharides. This will be done by using Benedict’s reagent and Iodine stain tests. Benedict’s reagent will react to reducing substances in the solution by oxidizing it and changing the structure of the reducing sugar to form a colored precipitate. The color of this precipitate can be used to determine the concentration of reducing sugars in the substance. If the precipitate is blue no reducing sugars are in the substance. If the precipitate is bluish green, green, yellow, or orange it does contain reducing sugars. Iodine stain will be used to determine if the solution contains polysaccharides.…

    • 1115 Words
    • 5 Pages
    Powerful Essays
  • Satisfactory Essays

    The laboratory exam will be given on Tuesday December 4th (sections 0375 and 3252) or…

    • 371 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    A few experiments were performed to test for the presence of the different types of macromolecules of various solutions. The iodine tested for starch and glycogen, Benedict’s test tested for reducing sugars for carbohydrates and biuret test was performed to test for proteins. It is necessary to understand how important controls are in a lab experiment. A control is where all of the experimental variables can be controlled and monitored. Results are determined by comparing these variables to the control. Without them, no results would be valid in the experiment. To tell whether or not the experimental procedure were carried out properly and correctly, treatment controls were used. They can either be positive or negative. Correct data with followed procedures, resulting in a working experiment would be a positive control. On the opposite hand, negative controls would give negative results if everything were to be followed in the experiment.…

    • 1158 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    SC121 Unit 2 Assignment 1

    • 545 Words
    • 2 Pages

    Discuss the various types of lipids found in the body and incorporate the following questions:…

    • 545 Words
    • 2 Pages
    Satisfactory Essays
  • Powerful Essays

    These biological macromolecules are to be identified by the changes in colour through three different tests - Iodine Test for starch and glycogen, Benedict 's Test for reducing sugars, and Biuret Test for Proteins. However, only two macromolecules are being identified in this experiment - carbohydrates and proteins. There are 12 solutions to be tested in this experiment. The Iodine test is used to indentify starch and glycogen in the given solutions. Of the 12 solutions, solution 8 is a starch solution and solution 7 is a glycogen solution. Starch solutions turn blue-black when Iodine solution is added to it. This is due to the formation of polyiodide chains when the Iodine solution mixes with starch. Starch contains both amylose and amylopectin. The amylose molecules in starch form helices at the locations where the Iodine molecules assemble. This cause a dark blue-black colour change ("Starch-iodine test", 2008). Therefore, solution 8 should turn blue-black when Iodine solution is added to it since it is a starch solution. However, glycogen solutions turn red-brown when Iodine solution is added. The chemical structure of glycogen is similar to the structure of amylopectin. Glycogen is highly branched. These branches are formed through acetal linkages. It is because of the highly branched structure of glycogen that solutions of glycogen turn red-brown in Iodine solutions (Ophardt, 2003). Thus,…

    • 2377 Words
    • 10 Pages
    Powerful Essays
  • Satisfactory Essays

    5 Day Food Analysis Paper

    • 280 Words
    • 2 Pages

    During the 5 day span I met the calorie requirement of 2850 in daily food intake due to my high activity level. I pretty much had a balanced diet in terms of having each food group represented in my daily intake. I plan on to keeping my muscle mass, so I pretty much had a diet plan before. But going through the calorie tracker in the website made me realize that I was eating too much junk food than intended. It really didn’t have any adverse on my physique due to my early mentioned level of activity but by cutting down on my junk food intake for the week I was recording this log I was able to enjoy good wholesome food, without spending a lot of money in college cafeteria. Not only was I able to do this I was able to feel…

    • 280 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Lab Abstract 1

    • 266 Words
    • 1 Page

    The purpose of this experiment is to learn how to test for common macromolecules and identify macromolecules in substances. The four tests that were used were: Sudan IV test, Iodine test, Biuret test, and Benedict’s test. The Sudan IV test identifies lipids. If the test is positive it will have a deep pink layer. Out of the four tested substances, only vegetable oil tested positive. The Iodine test determines if starch is present. In this test, potassium iodine reacts with starch molecules turning a blue or purple color. The starch substance was the only one tested to be positive for any of the substances. Benedict’s test is used to identify sugars. For this test, you add Benedict’s solution to a heated substance. If sugars are present, the substance will produce an orange or red color. The substances that tested positive were glucose, fructose, lactose, and Sprite. They tested positive because all of them had enough sugar to be detected. The Biuret test is used to detect proteins. A substance that contains two or more adjacent peptide bonds will form a purple complex. The higher number of bonds means a more intense color. Both Sprite and Half & Half tested positive. Although it seems these items wouldn’t contain proteins, they have just enough to be able to be detected. It was proved that various food contain a variety of macromolecules. Even though these tests worked, they’re not objective for trace substances. In the future, more sensitive testing agents should be used.…

    • 266 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    Spring 2015 Study Guide 1

    • 364 Words
    • 2 Pages

    Be familiar with the biochemical tests performed in lab-including the Millon test, Biuret test, Benedict’s test, Iodine test, Grease spot and Sudan Dye test. Know what each test is used for- protein (peptide bonds or tyrosine groups), glucose, sucrose, starch or lipids. You should know the chemicals/reagents in each test and the results that would indicate a positive or negative for each biochemical.…

    • 364 Words
    • 2 Pages
    Satisfactory Essays
  • Powerful Essays

    Biology Exam Paper

    • 2083 Words
    • 22 Pages

     Do all rough work in this book. Cross through any work you do not want…

    • 2083 Words
    • 22 Pages
    Powerful Essays
  • Good Essays

    Evaluation of Diets

    • 1028 Words
    • 5 Pages

    Although I researched several diets that are out there I wanted to write about the diet that I have been currently been on for the last 6 ½ years. Even though the name is called “The Abs Diet,” in my opinion this is really not a diet but adopting a new way of eating that makes sense. I am a big believer in this “diet” and since being on it I have been in the best shape of my life and also have more energy. This diet became famous by the editors of Women’s Health Magazine. This is also where I first heard of it and then picked up the book that is based on this diet. The premise of this diet is to not only get a flat stomach but also gain muscle and lose fat by never feeling deprived of foods. The first part of the book explains the “power foods” that you should eat and explains the benefits of each one. The second part is devoted to all sorts of recipes in order to consume the “power foods” and stay full. The last part focuses on exercise, some cardio, but mostly strength training. Like any fad diet, they promise something that may or may not be realistic. The claim is that the first 2 weeks you will be able to lose up to 12 pound of stomach fat. I am not sure if this is the case with everyone but I definitely saw a huge difference and have had visible abs in the last 6.5 years that I have been following their eating plan. The basics in the eating plan for this diet is that it is important to incorporate the 12 “power foods” everyday. The 12 foods are: almonds and nuts, spinach and green vegetables, beans and legumes, dairy that is low in fat, unsweetened oatmeal, natural or organic peanut butter, Lean meats like turkey, eggs, whole-grains, whey powder, olive oil, and raspberries and other berries. It says to also eat 6 times a day with 3 solid meals and 3 healthy snacks in between. The amount of calories you are consuming is not a huge focus on this diet but recommends around 1500 calories a day. The foods and meal chosen in this diet are emphasized as high…

    • 1028 Words
    • 5 Pages
    Good Essays