Quantitative Analysis of Vitamin C Contained in a Fruit Juice
Laboratory exercise 3 : Quantitative analysis of vitamin C contained in a fruit juice
Aim: To investigate the amount of vitamin C found in all kinds of fruit juice
Introduction :
Vitamin C is an antioxidant, along with vitamin E, beta-carotene, and many other plant-based nutrients. Antioxidants block some of the damage caused by free radicals, substances that damage DNA. The build-up of free radicals over time may contribute to the aging process and the development of health conditions such as cancer, heart disease, and arthritis.
It' s rare to be seriously deficient in vitamin C, although evidence suggests that many people may have low levels of vitamin C. Smoking cigarettes lowers the amount of vitamin C in the body, so smokers are at a higher risk of deficiency.
Signs of vitamin deficiency include dry and splitting hair; gingivitis (inflammation of the gums) and bleeding gums; rough, dry, scaly skin; decreased wound-healing rate, easy bruising; nosebleeds; and a decreased ability to ward off infection. A severe form of vitamin C deficiency is known as scurvy.
Source: http://www.umm.edu/altmed/articles/vitamin-c-000339.htm#ixzz2Na1A1oHh
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Apparatus and materials: As describe in MFHS101 biology practical guide
Method: As describe in MFHS101 biology practical guide
Ways to calculate Vitamin C content:
Volume if standard solution to decolorize DCPIP (ml) x Amount of Vitamin C in standard solution ( 1 Volume of fruit juice sample to decolourize DCPIP (ml) mg/ml)
Results:
Solution | Initial volume (ml) | Final volume (ml) | Volume of juice used (ml) | Mean volume of juice used (ml) | Amount of ascorbic acid in 1 ml sample | 0.1% ascorbic acid | 30 | 31 | 1 | 1 | 1 | | 31 | 32 | 1 | | | | 33 | 34 | 1 | | | Juice A | 32.1 | 34 | 1.9 | 1.6 | 0.63 | | 34 | 35.6 | 1.8 | | | | 35.6 | 36.9 | 1.3 | | | Juice B
Aim: To investigate the amount of vitamin C found in all kinds of fruit juice
Introduction :
Vitamin C is an antioxidant, along with vitamin E, beta-carotene, and many other plant-based nutrients. Antioxidants block some of the damage caused by free radicals, substances that damage DNA. The build-up of free radicals over time may contribute to the aging process and the development of health conditions such as cancer, heart disease, and arthritis.
It' s rare to be seriously deficient in vitamin C, although evidence suggests that many people may have low levels of vitamin C. Smoking cigarettes lowers the amount of vitamin C in the body, so smokers are at a higher risk of deficiency.
Signs of vitamin deficiency include dry and splitting hair; gingivitis (inflammation of the gums) and bleeding gums; rough, dry, scaly skin; decreased wound-healing rate, easy bruising; nosebleeds; and a decreased ability to ward off infection. A severe form of vitamin C deficiency is known as scurvy.
Source: http://www.umm.edu/altmed/articles/vitamin-c-000339.htm#ixzz2Na1A1oHh
Follow us: @UMMC on Twitter | MedCenter on Facebook
Apparatus and materials: As describe in MFHS101 biology practical guide
Method: As describe in MFHS101 biology practical guide
Ways to calculate Vitamin C content:
Volume if standard solution to decolorize DCPIP (ml) x Amount of Vitamin C in standard solution ( 1 Volume of fruit juice sample to decolourize DCPIP (ml) mg/ml)
Results:
Solution | Initial volume (ml) | Final volume (ml) | Volume of juice used (ml) | Mean volume of juice used (ml) | Amount of ascorbic acid in 1 ml sample | 0.1% ascorbic acid | 30 | 31 | 1 | 1 | 1 | | 31 | 32 | 1 | | | | 33 | 34 | 1 | | | Juice A | 32.1 | 34 | 1.9 | 1.6 | 0.63 | | 34 | 35.6 | 1.8 | | | | 35.6 | 36.9 | 1.3 | | | Juice B