Relative Water Measure Lab Report

Succulence was determined according to the following equation:
Succulence = Fresh Weight/Oven Dry Weight…………………........................................................(1) Measurement of Relative Water Content (RWC%);Relative Turgidity (RT %):
Relative water content was estimated according to a modification of the method of Weatherly (1950); Weatherly and Barr (1962); Slatyer (1957);Fletcher et al. (1988)on the final day of the experiment and was calculated by the formula given byKramer (1983).Detached leaf samples were weight immediately and floated on distilled water in a darkened refrigerator (5˚C). Saturation of the leaves was attained after 24 h. and the leaves were rapidly and thorough blotted and weighed immediately. The leaves were then dried at 80˚C to constant weight in an air -circulation oven to constant weight. The relative water content of leaves was expressed according to the following equation:
Lloyd's Strips (1908) taken from the leaves under investigation and immediately immersed in absolute alcohol for fixation and preservation. Three leaves from a treated plant were selected for uniformity of appearance and status of growth. From each of these leaves, two epidermal strips were token and on each strip two areas of about 0.25 m2 were selected for determining three stomatal counts in each upper and lower epidermis. For each determination three strips were taken from three different leaves of about the same age, ten stomata were chosen at random from each strip, and their widths were determined by means of a standardized ocular micrometer. The number of stomata per mm2 (Stomatal Frequency) on upper and lower epidermis was determined using the square ocular