Preview

Remazol Red Dye Case Study

Better Essays
Open Document
Open Document
1579 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Remazol Red Dye Case Study
The release of dyes through contaminated water into the ecosystem from various industries like textile, leather, cosmetic, paper, pharmaceutical and food industries is not only a source of aesthetic pollution but also can cause human health disorders and adversely affects the aquatic life causing severe environmental problems worldwide. In view of increasing concern from perspective of environmental safety and health, physico-chemical and biological techniques are to be constantly explored for decolourization and degradation of dyes.
Remazol red RB dye was selected as model azo dye for the study. It is known to be toxicity, mutagenic and carcinogenic in nature because of the presence of the aromatic amine group (Zollinger, 2003; Jalandoni-Buan
…show more content…
Under static conditions, decolourization of remazol red RB dye by bacterial isolates was more efficient than at shaking condition within 24 hours. In static condition transfer of oxygen is limited to the broth surface and the cell cultures will most likely sediment to the bottom of the flasks and become rapidly oxygen - depleted (Stolz, 2001; Chen, 2002).The reason for reduced decolourization at shaking condition could be due to the competition in oxidation of reduced electron carriers with either oxygen or azo groups as the electron acceptor (Mabrouk and Yusef, 2008; Dawkar et al., 2010). Microbial growth and bacterial decolourization are independent for decolourization of dyes as azoreductase is repressed under aerobic conditions (Chang et al., 2004).The data suggest that in static condition is more appropriate for the decolourization of the dye by the bacterium. Similar results were reported by bacterial strains are Shewanella putrefaciens AS96, Comamonas sp.UVS, Bacillus subtilis, Vibrio harveyi TEMS1 and Pseudomonas sp. SUK1. All these strains have shown promising results for dye degradation under static conditions (Khalid et al., 2008; Jadhav et al., 2008; Gurulakshmi et al., 2008; Ozdemir et al., 2008; Kalyani et al., 2009). Decolourization of azo dyes cannot take place in extreme anaerobic conditions that is under oxygen-free nitrogen sparging/gas flushing. This clearly implies that bacteria needs small amount of oxygen to maintain basic cellular activity for decolourization (Chen,

You May Also Find These Documents Helpful

  • Powerful Essays

    Food Dyes Lab

    • 545 Words
    • 3 Pages

    To complete this lab, I first mixed the solvent solution consisting of the proper ratio of water, salt, and isopropyl alcohol in a Pyrex measuring cup. I then prepared my chromatography papers for 2 trials by drawing, with a pencil, the appropriate lines and labels. Using a toothpick, I added the color to the paper, and then repeated until the color was strong. I repeated this step several more times for the M&Ms as the color solution was not as strong as the food dyes. After the dyes were dry, I rolled the paper and added it to the pie plate, which contained a small amount of the prepared solvent. I then waited for the solution to…

    • 545 Words
    • 3 Pages
    Powerful Essays
  • Satisfactory Essays

    AP Chemistry Kinetics Lab

    • 335 Words
    • 2 Pages

    In this experiment, rate laws and graphs help calculate the oxidation of food dyes by sodium hypochlorite. During the experiment the linear relationship between absorbance…

    • 335 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    bio 260

    • 428 Words
    • 2 Pages

    What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure if the alcohol step in the gram stain was omitted?…

    • 428 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    The use of red dye #40 is common in various soft drinks today. The labels on these beverages do not specify how much dye we are consuming. We did this experiment to find out which soda uses the most dye. Using a spectrophotometer, we measured how much light is absorbed by various known concentrations of red dye. After collecting this data, a standard curve was made that correlated the concentration of red dye #40 to its absorbance rate. Our results showed that the sample of strawberry crush had the highest concentration of red dye #40 when compared to grape soda, diet cherry, and big red. After doing this experiment, one knows how much red dye #40 he or she is consuming when drinking these products.…

    • 342 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Food Dyes Lab

    • 671 Words
    • 3 Pages

    To identify the dyes present a sample of the beverage was tested in a spectrometer, the highest absorption rates were found to be at 503.7 nm (A=0.281) and 630.7 nm (A=0.270). This lead to the conclusion that the beverage contained red 40 and blue 1. To determine the concentration of the dyes a series of dilutions was prepared for both dyes and tested with the spectrometer. This data was converted into point graphs and a trendline was established. The slope of the trendline (y=mx) was then multiplied by the Absorbance of the associated dye to find the concentration. The final goal was to replicate the beverage using the data gathered. To accomplish this a solution was designed consisting of 15.34% red 40 and 14.12% blue…

    • 671 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Micro lab quiz 2

    • 1967 Words
    • 8 Pages

    Simple dyes used to stain bacteria have a positive charge cationic (+), therefore, they are attracted to the bacteria that are usually negatively charged anionic (-)…

    • 1967 Words
    • 8 Pages
    Better Essays
  • Good Essays

    Spectrophotometry

    • 1506 Words
    • 7 Pages

    Spectrophotometry was used in the lab to determine whether non-magnetized zeolite, magnetized zeolite, or charcoal was the more effective sequestration agent for Procion Red Dye. A calibration curve was created with the known concentrations and the absorbances of the Procion Red Dye dilutions at λmax. The slope of the calibration curve was used to determine the concentration of the analytes. As a result, charcoal was shown to be the more effective sequestration agent.…

    • 1506 Words
    • 7 Pages
    Good Essays
  • Good Essays

    In this lab using the bag of FD&C dyes, bag of M&M’s, 2 packs of Kool-Aid , and a pack of Clover Valley store brand food coloring I am going to place a drop of each dye at the bottom of the chromatography paper. For the experiment with the rubbing alcohol I also placed a drop of each dye, but used a paper coffee filter. In order to get the dye color extracted from the M&M’s and Kool-Aid I added a little additional water.…

    • 599 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Chem Lab Chromatography

    • 558 Words
    • 3 Pages

    Discussion of Theory: Chromatography, resolution and selectivity played major roles in making the experiment work. The experiment demonstrated a common use of chromatography, and works to explain a way to separate mixtures. The dyes were successfully separated and analyzed in the cartridge.…

    • 558 Words
    • 3 Pages
    Good Essays
  • Good Essays

    3.03 Calorimetry Lab

    • 681 Words
    • 3 Pages

    Although not all water can be used for drinking purposes, in many places, water that is drinkable- is polluted or deluded by many agricultural and industrial motives and damage humans have caused (Friend). In this experiment, the goal is not to purify muddy water completely from microorganisms and bacteria, but to find the most efficient way to cleanse it from the dirt and soil particles that cause the murky color using physical…

    • 681 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Red Dye Lab

    • 1247 Words
    • 4 Pages

    Citations: 2009. Uptake of neutral red dye by Saccharomyces cerevisiae in the presence of a…

    • 1247 Words
    • 4 Pages
    Powerful Essays
  • Good Essays

    Here is what to look for on the packaging to make sure your dyes are safe:…

    • 1694 Words
    • 7 Pages
    Good Essays
  • Satisfactory Essays

    Essay On Cosmetology

    • 359 Words
    • 2 Pages

    All hair dye has a ‘main ingredient’ and that is p-diamines or p-aminophenols which are both oxidized by hydrogen peroxide that react to produce dyes. Bleaching, is a way to remove color by lightening the hair. Bleach reacts with the melanin to remove the color away causing an irreversible chain reaction. Although the melanin is still there the oxidized molecules are…

    • 359 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    5.05 Chemistry

    • 841 Words
    • 3 Pages

    Purpose: The purpose of this experiment is to compare the separation abilities of salt water and isopropyl alcohol using paper and candy pigments.…

    • 841 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Hair Dye Research Paper

    • 664 Words
    • 3 Pages

    In this report, you’ll read about hair dye and its negative side effects. The first section of this report I explain the three main types of hair dyes. Next, the negative side effects of hair dye products are mentioned and elaborated. After that, safety tips and precautions are given. Finally, the conclusion will tie it all together. This is my research report on how hair dye products can cause various, life threatening side effects, like cancer, after use.…

    • 664 Words
    • 3 Pages
    Good Essays