Research in Cloning Summary..
**INTRODUCTION**
What is cloning?
Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. It describes the processes used to create an exact genetic replica of another cell, tissue or organism. The copied material, which has the same genetic makeup as the original, is referred to as a clone.
When is cloning done?
Cloning is done to amplify the DNA fragments containing the gene of interest and ensures pure sample of gene. When the concentration of the required gene products (proteins) is very low, the production of multiple clones makes it easy to obtain large quantity of the gene products.
What all can be cloned?
Cloning is a powerful techninque that can be implemented to obtain clones of genes (molecular cloning), cells (cell cloning) and organisms(organism cloning). There are three different types of cloning: Gene cloning, which creates copies of genes or segments of DNA Reproductive cloning, which creates copies of whole animals Therapeutic cloning, which creates embryonic stem cells. Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body.
What are the requisites for cloning?
In biotechnology, cloning requires a gene of interest, a vehicle (vectors) to carry the gene, a host to provide an environment for multiplication of genes and a medium for growth of the host strain.
What is DNA cloning?
DNA cloning is the procedures to produce multiple copies of a single gene or segment of DNA. a DNA fragment containing the gene of interest is isolated from chromosomal DNA using restriction enzymes and then united with a plasmid that has been cut with the same restriction enzymes. When the fragment of chromosomal DNA is joined with its cloning vector in the lab, it is called a "recombinant DNA molecule." Following introduction into suitable host cells, the recombinant DNA can then be reproduced along
What is cloning?
Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. It describes the processes used to create an exact genetic replica of another cell, tissue or organism. The copied material, which has the same genetic makeup as the original, is referred to as a clone.
When is cloning done?
Cloning is done to amplify the DNA fragments containing the gene of interest and ensures pure sample of gene. When the concentration of the required gene products (proteins) is very low, the production of multiple clones makes it easy to obtain large quantity of the gene products.
What all can be cloned?
Cloning is a powerful techninque that can be implemented to obtain clones of genes (molecular cloning), cells (cell cloning) and organisms(organism cloning). There are three different types of cloning: Gene cloning, which creates copies of genes or segments of DNA Reproductive cloning, which creates copies of whole animals Therapeutic cloning, which creates embryonic stem cells. Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body.
What are the requisites for cloning?
In biotechnology, cloning requires a gene of interest, a vehicle (vectors) to carry the gene, a host to provide an environment for multiplication of genes and a medium for growth of the host strain.
What is DNA cloning?
DNA cloning is the procedures to produce multiple copies of a single gene or segment of DNA. a DNA fragment containing the gene of interest is isolated from chromosomal DNA using restriction enzymes and then united with a plasmid that has been cut with the same restriction enzymes. When the fragment of chromosomal DNA is joined with its cloning vector in the lab, it is called a "recombinant DNA molecule." Following introduction into suitable host cells, the recombinant DNA can then be reproduced along