Results and Discussion Example
Results
In this experiment we used paper chromatography to determine the amount of chlorophyll a, chlorophyll b, xanthophyll, and beta-carotene in a tube of blended leaf extract. We recorded our findings on the table labeled table 1. Table 1 shows the transmittance at each wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used; with the help of acetone we saw the spectrum and the differences of the different pigments. Percentage transmittance in Table 1 show that the lower the number, the more dense that solution is at that specific wavelength. As the numbers get higher, the less dense it is and if the number is 100 at a specific wavelength then the solution was clear. As we can see from Table 1, each of the pigments had different low transmittance based on how high the absorption was in each of the pigments. For this we calculated that at there was a peak on the graph at the lowest points in table 1. For instance, in the chlorophyll a column in Table 1 the lowest points on the graphs at 8 and 28 and also at 44, which shows that there will be a peak around those points. In chlorophyll b, the lowest points are from 25.2, 12.4, and 9.4, which would be the first peak and the next will be at 55.6. In Xanthophyll, the points were the first peak will be are 52.4, 43.6, 44.8, and 53.0. Lastly in Carotene, the graph will peak at 92.2, 93.6, and 92.4. By using these points we predicted the behavior of the graph. TABLE 1 | | | Wavelength | Chlorophyll a | Chlorophyll b | Xanthophyll | Carotene | 400 | 12 | 42 | 61.8 | 96.8 | 420 | 8 | 25.2 | 52.4 | 99.6 | 440 | 28 | 12.4 | 43.6 | 92.2 | 460 | 71.5 | 9.4 | 44.8 | 93.6 | 480 | 81 | 48.4 | 53 | 92.4 | 500 | 88 | 88.6 | 83.2 | 99.2 | 520 | 88 | 91.4 | 94.2 | 100 | 540 | 84.5 | 87.4 | 95.6 | 100 | 560 | 83 | 85.2 | 96.4 | 99.2 | 580 | 71.5 | 83.4 | 96.2 | 100 | 600 |
In this experiment we used paper chromatography to determine the amount of chlorophyll a, chlorophyll b, xanthophyll, and beta-carotene in a tube of blended leaf extract. We recorded our findings on the table labeled table 1. Table 1 shows the transmittance at each wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used; with the help of acetone we saw the spectrum and the differences of the different pigments. Percentage transmittance in Table 1 show that the lower the number, the more dense that solution is at that specific wavelength. As the numbers get higher, the less dense it is and if the number is 100 at a specific wavelength then the solution was clear. As we can see from Table 1, each of the pigments had different low transmittance based on how high the absorption was in each of the pigments. For this we calculated that at there was a peak on the graph at the lowest points in table 1. For instance, in the chlorophyll a column in Table 1 the lowest points on the graphs at 8 and 28 and also at 44, which shows that there will be a peak around those points. In chlorophyll b, the lowest points are from 25.2, 12.4, and 9.4, which would be the first peak and the next will be at 55.6. In Xanthophyll, the points were the first peak will be are 52.4, 43.6, 44.8, and 53.0. Lastly in Carotene, the graph will peak at 92.2, 93.6, and 92.4. By using these points we predicted the behavior of the graph. TABLE 1 | | | Wavelength | Chlorophyll a | Chlorophyll b | Xanthophyll | Carotene | 400 | 12 | 42 | 61.8 | 96.8 | 420 | 8 | 25.2 | 52.4 | 99.6 | 440 | 28 | 12.4 | 43.6 | 92.2 | 460 | 71.5 | 9.4 | 44.8 | 93.6 | 480 | 81 | 48.4 | 53 | 92.4 | 500 | 88 | 88.6 | 83.2 | 99.2 | 520 | 88 | 91.4 | 94.2 | 100 | 540 | 84.5 | 87.4 | 95.6 | 100 | 560 | 83 | 85.2 | 96.4 | 99.2 | 580 | 71.5 | 83.4 | 96.2 | 100 | 600 |