review sheet 12 a&p
REVIEW SHEET
EXERCISE 12
Serological Testing
NAME:
LAB TIME/DATE:
Positive and Negative Controls
1. Why are there a number of washing steps in serological tests? They are needed in order to remove any non specific binding that may happen.
2. Describe how you would know that you had a “false positive” result. What does this mean for the rest of your results? If it has a negative control then it’s a false positive so the results are invalidated.
3. Describe how you would know that you had a “false negative” result. What does this mean for the rest of your results?
Negative result with a positive control is a false negative so the results are not true.
Direct Fluorescent Antibody Technique
4. Why is this technique a direct method? Because of the comjugation of an antibody with flurescent dye
5. What is an elementary body? Its an infectious particle of any of several microorganisms.
6. How do elementary bodies look under the fluorescent microscope? Red, with different concaved shapes with a green spot in them almost like a nucleus looking thing.
7. What do you think would happen if you did not fix the sample to the slide with 95% ethyl alcohol?
If we would not have use alcohol then I’m guessing the slide would not have turned out like it did. Maybe it would have washed away.
8. Which patient(s) tested positive for Chlamydia? Patient a
9. Was there any nonspecific binding for any of the samples? Explain. Patient c maybe because there was a nonspecific binding.
Ouchterlony Technique
10. What is a precipitin line?
11. What is the unknown antigen in the simulation?
12. Considering your results, do you think that human serum albumin and bovine serum albumin have epitopes in common? Explain.
13. What is the process resulting in antigen and antibody moving toward each other?
Enzyme-Linked Immunosorbent Assay
14. In the “sandwich” analogy of the direct ELISA, what is the
EXERCISE 12
Serological Testing
NAME:
LAB TIME/DATE:
Positive and Negative Controls
1. Why are there a number of washing steps in serological tests? They are needed in order to remove any non specific binding that may happen.
2. Describe how you would know that you had a “false positive” result. What does this mean for the rest of your results? If it has a negative control then it’s a false positive so the results are invalidated.
3. Describe how you would know that you had a “false negative” result. What does this mean for the rest of your results?
Negative result with a positive control is a false negative so the results are not true.
Direct Fluorescent Antibody Technique
4. Why is this technique a direct method? Because of the comjugation of an antibody with flurescent dye
5. What is an elementary body? Its an infectious particle of any of several microorganisms.
6. How do elementary bodies look under the fluorescent microscope? Red, with different concaved shapes with a green spot in them almost like a nucleus looking thing.
7. What do you think would happen if you did not fix the sample to the slide with 95% ethyl alcohol?
If we would not have use alcohol then I’m guessing the slide would not have turned out like it did. Maybe it would have washed away.
8. Which patient(s) tested positive for Chlamydia? Patient a
9. Was there any nonspecific binding for any of the samples? Explain. Patient c maybe because there was a nonspecific binding.
Ouchterlony Technique
10. What is a precipitin line?
11. What is the unknown antigen in the simulation?
12. Considering your results, do you think that human serum albumin and bovine serum albumin have epitopes in common? Explain.
13. What is the process resulting in antigen and antibody moving toward each other?
Enzyme-Linked Immunosorbent Assay
14. In the “sandwich” analogy of the direct ELISA, what is the