Serological testing
Using Direct Fluorescent Antibody Technique to Test for Chlamydia
1. Describe the importance of the washing steps in the direct antibody fluorescence test.
It’s very important for the washing steps in the direct antibody fluorescence test to decrease the no-specific binding.
2. Explain where the epitope (antigenic determinant) is located.
The epitopes are located in the antigens for the antibodies binding.
3. Describe how a positive result is detected in this serological test.
The elementary bodies of the Chlamydia trachomatis stains green inside the red host cell, and the presence of more elementary bodies in a field of view compared to the positive control is considered a positive result.
4. How would the results be affected if a negative control gave a positive result?
If a negative control gave a positive result, the positive results would be underestimated.
A C T I V I T Y 2
Comparing Samples with Ouchterlony Double Diffusion
1. Describe how you were able to determine what antigen is in the unknown well.
The results between well 2 & 5 were the same results as 3 & 4. We know that human serum albumin was placed in 4, so it should be the same in 5 to achieve the same result.
2. Why does the precipitin line form?
The precipitin line occurs when the antigen and antibody are in optimal proportions and cross-linking occurs forming an insoluble precipitate.
3. Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction?
I think they have epitopes in common for the results showed they have partial identity. Same as my prediction.
1. Describe the importance of the washing steps in the direct antibody fluorescence test.
It’s very important for the washing steps in the direct antibody fluorescence test to decrease the no-specific binding.
2. Explain where the epitope (antigenic determinant) is located.
The epitopes are located in the antigens for the antibodies binding.
3. Describe how a positive result is detected in this serological test.
The elementary bodies of the Chlamydia trachomatis stains green inside the red host cell, and the presence of more elementary bodies in a field of view compared to the positive control is considered a positive result.
4. How would the results be affected if a negative control gave a positive result?
If a negative control gave a positive result, the positive results would be underestimated.
A C T I V I T Y 2
Comparing Samples with Ouchterlony Double Diffusion
1. Describe how you were able to determine what antigen is in the unknown well.
The results between well 2 & 5 were the same results as 3 & 4. We know that human serum albumin was placed in 4, so it should be the same in 5 to achieve the same result.
2. Why does the precipitin line form?
The precipitin line occurs when the antigen and antibody are in optimal proportions and cross-linking occurs forming an insoluble precipitate.
3. Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction?
I think they have epitopes in common for the results showed they have partial identity. Same as my prediction.