RFLP
Bulletin of Entomological Research (2007) 97, 299–308
doi:10.1017/S000748530700507X
Genetic variation among Mediterranean populations of Sesamia nonagrioides
(Lepidoptera: Noctuidae) as revealed by
RFLP mtDNA analysis
J.T. Margaritopoulos1 *, B. Gotosopoulos1, Z. Mamuris2,
P.J. Skouras1, K.C. Voudouris1, N. Bacandritsos3,
A.A. Fantinou4 and J.A. Tsitsipis1
1
Laboratory of Entomology and Agricultural Zoology, Department of
Agriculture, Crop Production and Rural Environment, University of
Thessaly, Fytokou Str., 38446 Nea Ionia, Magnesia, Greece: 2Department of
Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26,
41221 Larissa, Greece: 3Institute of Veterinary Research of Athens,
Neapoleos 25, Agia Paraskevi, 15310 Athens, Greece: 4Laboratory of
Ecology and Environmental Sciences, Agricultural University of Athens,
Iera Odos 75, 11855 Athens, Greece
Abstract
Restriction fragment length polymorphism analysis of two segments of mitochondrial DNA (COI and 16S rRNA) was used to examine genetic variation
`
in Sesamia nonagrioides (Lefebvre) populations from the Mediterranean basin.
Four populations were collected from central and southern Greece, and five from northern latitudes: Greece, Italy, France and Spain. No variation was observed in
COI, while 16S rRNA segment proved highly polymorphic and 28 different haplotypes were found. Lower intra-population polymorphism was found in the northern populations than in southern ones. Although no significant isolation by distance was found, the UPGMA tree based on Nei’s raw number of nucleotide differences separated the populations into two major groups, i.e. one with the northern (40.6 N–43.4 N) and the other with the southern populations
(37.3 N–39.2 N). Analysis of molecular variance revealed that most of the variation was between the two major groups (WCT = 0.559), and all pairwise comparisons between the northern and southern populations resulted in high and
doi:10.1017/S000748530700507X
Genetic variation among Mediterranean populations of Sesamia nonagrioides
(Lepidoptera: Noctuidae) as revealed by
RFLP mtDNA analysis
J.T. Margaritopoulos1 *, B. Gotosopoulos1, Z. Mamuris2,
P.J. Skouras1, K.C. Voudouris1, N. Bacandritsos3,
A.A. Fantinou4 and J.A. Tsitsipis1
1
Laboratory of Entomology and Agricultural Zoology, Department of
Agriculture, Crop Production and Rural Environment, University of
Thessaly, Fytokou Str., 38446 Nea Ionia, Magnesia, Greece: 2Department of
Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26,
41221 Larissa, Greece: 3Institute of Veterinary Research of Athens,
Neapoleos 25, Agia Paraskevi, 15310 Athens, Greece: 4Laboratory of
Ecology and Environmental Sciences, Agricultural University of Athens,
Iera Odos 75, 11855 Athens, Greece
Abstract
Restriction fragment length polymorphism analysis of two segments of mitochondrial DNA (COI and 16S rRNA) was used to examine genetic variation
`
in Sesamia nonagrioides (Lefebvre) populations from the Mediterranean basin.
Four populations were collected from central and southern Greece, and five from northern latitudes: Greece, Italy, France and Spain. No variation was observed in
COI, while 16S rRNA segment proved highly polymorphic and 28 different haplotypes were found. Lower intra-population polymorphism was found in the northern populations than in southern ones. Although no significant isolation by distance was found, the UPGMA tree based on Nei’s raw number of nucleotide differences separated the populations into two major groups, i.e. one with the northern (40.6 N–43.4 N) and the other with the southern populations
(37.3 N–39.2 N). Analysis of molecular variance revealed that most of the variation was between the two major groups (WCT = 0.559), and all pairwise comparisons between the northern and southern populations resulted in high and
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