Preview

Snare Complexes and Their Important Role with Vesicle Fusion and Docking

Powerful Essays
Open Document
Open Document
1167 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Snare Complexes and Their Important Role with Vesicle Fusion and Docking
biO315
Final Draft
SNARE Complexes and their Important Role with Vesicle Fusion and Docking
SNARE complex’s play a role in all vesicle fusion within the cell including going from the endoplasmic reticulum to the golgi body and golgi body to lysosomes or to mitigate exocytosis of intracellular vesicles with the cellular membrane. The SNARE complex is made up of 2 classes of protiens that allow vesicle fusion called the v-snares and t-snares. The v-snares are found on the vesicle and include synaptobrevin. The t-snares are located on the target membrane and has two components called syntaxin and SNAP-25 (Karp, 2008). The SNARE proteins are fixed tightly within the vesicle and target membrane by transmembrane domains. When the target membrane and vesicle come into contact and dock with each other the SNARE motifs composed of four alpha helices interact with each other and make a strong interwoven linkage (Wu et al., 2012). The zipper hypothesis says that the linkage of the t-snare and v-snare start assembling from the vesicle membrane and continue to intertwine or “zipper” down to the target membrane (Knecht and Grubmüller, 2003). The alpha helices pull tightly to each other causing the membranes to come in close proximity and membrane fusion is stimulated. The paper, All three components of the neuronal SNARE complex to secretory vesicle docking, by Wu et al., wanted to know how synaptobrevin, SNAP-25, and syntaxin were involved with vesicle and target membrane docking. In the paper they performed a number of experiments to test how SNARE proteins contributed to docking.
To determine if the v-snare synaptobrevin has a role to play in vesicle docking the amount of functional synaptobrevin was reduced via the addition of the neurotoxin botulinum (BoNT/d) which was labeled with an in-vector marker called DsRed into the cells. The neurotoxin botulinum (BoNT/d) cleaves the v-snare synaptobrevin. An Immunoblot experiment was performed to make sure the

You May Also Find These Documents Helpful

  • Good Essays

    Nsci 280 Week 4 Quiz

    • 756 Words
    • 4 Pages

    7. In excitation-contraction coupling, a. calcium ions must bind with myosin to expose active sites on actin. b. myosin heads bind to exposed active sites on actin. c. cross-bridges form between myosin heads and calcium ions. d. movement of the troponin-tropomyosin complex causes actin myofilaments to slide.…

    • 756 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    When an impulse arrives at an axon terminal, the vesicles release the neurotransmitters into the synaptic cleft. The neurotransmitter molecules diffuse across the synaptic cleft and attach themselves to receptors on the membrane of the neighboring cell. This stimulus causes positive sodium ions to rush across the cell membrane, stimulating the second cell. If the stimulation exceeds the cell’s threshold, a new impulse begins.…

    • 1017 Words
    • 5 Pages
    Powerful Essays
  • Satisfactory Essays

    Sliding Filament Theory

    • 444 Words
    • 2 Pages

    2. The action potential, carried into the cell via the T-tubules, causes the SR to release…

    • 444 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    What molecule must bind to the myosin head in order for it to disconnect with…

    • 283 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Homework 3

    • 830 Words
    • 4 Pages

    When the secretory vesicles opened to allow acetylcholine to enter the synaptic cleft Molecule X rushed into the synaptic cleft. Molecule X moved faster than Acetylcholine therefore binding to the receptor sites first.…

    • 830 Words
    • 4 Pages
    Satisfactory Essays
  • Satisfactory Essays

    1. Name each of the three primary embryonic brain vesicles. Use clinical terminology to name the resulting adult brain region.…

    • 337 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Glandular Epithelial Tissue

    • 7418 Words
    • 30 Pages

    Secretor vesicles only exist transiently in the cell and are part of the end membrane system that shuttle newly synthesized proteins within the…

    • 7418 Words
    • 30 Pages
    Good Essays
  • Better Essays

    The goal of the experiment was to better understand kinesin facilitated transport of vesicles and to observe the effects of mutations in nerve transmission in Caenorhabditis elegans. C. elegans is a species of nematode that has a simple nervous system and has synaptic vesicles that can be easily tagged with green fluorescent protein (GFP) and viewed under fluorescence microscopy. The experiment was to observe three strains of C. elegans and determine which of the phenotypes observed showed certain mutations and then do a comparison…

    • 1305 Words
    • 6 Pages
    Better Essays
  • Satisfactory Essays

    c. binding and fusion of HIV into target cell takes place by interaction with 2 membrane glycoproteins (GP)…

    • 2667 Words
    • 10 Pages
    Satisfactory Essays
  • Powerful Essays

    The terminal buttons of the presynaptic neuron hold the synaptic vesicles. These sacs filled with neurotransmitters pass…

    • 1592 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    synapse then drops neurotransmitter into the synaptic cleft between the first neuron’s synapse and the…

    • 710 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Chap009

    • 4787 Words
    • 22 Pages

    3. In synaptic signaling, neurotransmitters are released into a space that is referred to as a:…

    • 4787 Words
    • 22 Pages
    Satisfactory Essays
  • Good Essays

    3. Exocytosis of neurotransmitter from the axon terminal is triggered by an increase in the intracellular concentration of You correctly answered: c. Ca2+ .…

    • 604 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Ebola Timeline

    • 279 Words
    • 2 Pages

    | The team identified the Niemann-Pick C1 (NPC1) gene as one critical entry factor as well as the members of a complex called HOPS (short for homotypic fusion and vacuole protein sorting). Both are involved in enabling small membrane-bound sacs inside cells, called endosomes, to fuse with structures called lysosomes that are involved in degrading substances within cells.…

    • 279 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Botulism

    • 8613 Words
    • 35 Pages

    The recorded history of botulism begins in 1735, when the disease was first associated with German sausage (food-borne disease or food poisoning after eating sausage). In 1870, a German physician by the name of Muller derived the name botulism from the Latin word for sausage. Clostridium botulinum bacteria were first isolated in 1895, and a neurotoxin that it produces was isolated in 1944 by Dr. Edward Schantz. From1949 to the 1950s, the toxin (named BoNT A) was shown to block neuromuscular transmissions by blocking the release of acetylcholine from motor nerve endings. Botulism toxin(s) are some of the most toxic substances known to man; while the toxin has been considered for use as a biological weapon, it has also been used to treat many medical conditions. In 1980, Dr. Scott used the toxin to treat strabismus (deviation of the eye), and in December 1989, BoNT-A (BOTOX) was approved by the U.S. Food and Drug Administration (FDA) for the treatment of strabismus, blepharospasm, and hemifacial spasm in young patients. The use of BOTOX to treat glabellar lines (wrinkles and…

    • 8613 Words
    • 35 Pages
    Better Essays