Spectroscopy and Distilled Water
EXPERIMENT 2 : UV-VISIBLE DETERMINATION OF AN UNKNOWN CONCENTRATION OF KMnO4
OBJECTIVE
1. To determine the maximum wavelength of potassium permanganate.
2. To plot the calibration curve of potassium permanganate.
3. To determine the concentration of an unknown solution of potassium permanganate.
APPARATUS
Beaker, burette, glass rod ,volumetric flask 100ml ,dropper
CHEMICALS
Potassium permanganate (KMnO4), distilled water
PROCEDURE
A. Preparation of the KMnO4 standard solution
1. KMnO4 was weigh accurately to nearest mg. The reading was recorded. The solid transfer to a 100ml volumetric flask using funnel.
2. The solid was dissolved with distilled water. The flask was shaken after using stopper. Distilled water was added to the mark ,the dropper was used when reaching the last drop. The flask was stopped using stopper and shake several time to give the solution homogenize.
3. The stock was pour into a beaker. The beaker was labelled as 100 ppm.
4. 5.00 ml of the stock was pipet and diluted with distilled water in a 100ml volumetric flask.
5. The solution was transfer to a beaker and labelled as 5 ppm.
6. Steps 4 was repeated using 10ml, 15ml, and 20ml.
7. The beaker was labelled as 10ppm, 15ppm, and 20ppm respectively.
B. Preparation of the unknown
1. The unknown solution was pipet between 5.00ml to 20.00ml of the stock KMnO4 solution and was diluted with distilled water in a 100ml volumetric flask.
2. The solution was transfer to a beaker and labelled as unknown solution.
C. Determination of absorption maximum
1. Distilled water was used as blank for determination of absorption maximum.
2. The blank solution was put at the end of the tips contain quartz to calculate the absorption maximum.
3. The result of absorption maximum from the graph was printed.
4. Absorption maximum will be used to determine the unknown concentration. D. Operation of the UV-VIS spectrophotometer
1. Cary win UV was selected and
OBJECTIVE
1. To determine the maximum wavelength of potassium permanganate.
2. To plot the calibration curve of potassium permanganate.
3. To determine the concentration of an unknown solution of potassium permanganate.
APPARATUS
Beaker, burette, glass rod ,volumetric flask 100ml ,dropper
CHEMICALS
Potassium permanganate (KMnO4), distilled water
PROCEDURE
A. Preparation of the KMnO4 standard solution
1. KMnO4 was weigh accurately to nearest mg. The reading was recorded. The solid transfer to a 100ml volumetric flask using funnel.
2. The solid was dissolved with distilled water. The flask was shaken after using stopper. Distilled water was added to the mark ,the dropper was used when reaching the last drop. The flask was stopped using stopper and shake several time to give the solution homogenize.
3. The stock was pour into a beaker. The beaker was labelled as 100 ppm.
4. 5.00 ml of the stock was pipet and diluted with distilled water in a 100ml volumetric flask.
5. The solution was transfer to a beaker and labelled as 5 ppm.
6. Steps 4 was repeated using 10ml, 15ml, and 20ml.
7. The beaker was labelled as 10ppm, 15ppm, and 20ppm respectively.
B. Preparation of the unknown
1. The unknown solution was pipet between 5.00ml to 20.00ml of the stock KMnO4 solution and was diluted with distilled water in a 100ml volumetric flask.
2. The solution was transfer to a beaker and labelled as unknown solution.
C. Determination of absorption maximum
1. Distilled water was used as blank for determination of absorption maximum.
2. The blank solution was put at the end of the tips contain quartz to calculate the absorption maximum.
3. The result of absorption maximum from the graph was printed.
4. Absorption maximum will be used to determine the unknown concentration. D. Operation of the UV-VIS spectrophotometer
1. Cary win UV was selected and