synthesis of silver nanaoparticles
SYNTHESIS OF SILVER NANOPARTICLES AND ITS CYTOTOXIC
EFFECT AGAINST THP-1 CANCER CELL LINE
1
*
Department of Biotechnology, Modern College, Shivajinagar, University of Pune, Pune, India.
Mrs Rebecca Thombre
email
rebecca.thombre@gmail.com
Telephone
9822224957
020 400966725
ABSTRACT:
Nanotechnology is a field of science which involves the synthesis and applications of nanoparticles.
Silver nanoparticles (AgNp) have applications in various fields due to their unique optical, physical, electrical and medicinal properties. In this study, silver nanoparticles were synthesized by biophysical method. Silver nanoparticles were characterized using UV-Visible absorption spectroscopy (Surface Plasmon absorption at 418-420 nm), Energy Dispersive Spectroscopy
(EDX), Scanning Electron Microscopy (SEM) and X- ray diffraction (XRD). The cytotoxic effect of silver nanoparticles on acute myeloid leukemia cell line (THP – 1 cell line) was studied in vitro.
The activity of chemotherapeutic drugs Cyclophosphamide and Busulfan was enhanced in presence of silver nanoparticles whereas Mercaptopurine was found to be more effective when used individually against the THP- 1 cell line.
KEYWORDS:
Silver nanoparticles, Anti-cancerous property, Cyclophosphamide, Mercaptopurine, Busulfan
INTRODUCTION:
Nanotechnology is a promising arena and has exposed a panorama of applications in industrial, mechanical and medical field. In general nanoparticles are defined as particles with size < 100nm.
Various methods are used for the synthesis of metallic silver nanoparticles like chemical 1, electrochemical 2, laser ablation 5, photochemical 4 and biological, γ-radiation 3. Among all metallic nanoparticles, silver (Ag) nanoparticles are used in diverse fields because of their unique physical, chemical as well as their anti cancerous property
6
and antimicrobial
7
Silver nanoparticles are
generally produced by chemical methods
EFFECT AGAINST THP-1 CANCER CELL LINE
1
*
Department of Biotechnology, Modern College, Shivajinagar, University of Pune, Pune, India.
Mrs Rebecca Thombre
rebecca.thombre@gmail.com
Telephone
9822224957
020 400966725
ABSTRACT:
Nanotechnology is a field of science which involves the synthesis and applications of nanoparticles.
Silver nanoparticles (AgNp) have applications in various fields due to their unique optical, physical, electrical and medicinal properties. In this study, silver nanoparticles were synthesized by biophysical method. Silver nanoparticles were characterized using UV-Visible absorption spectroscopy (Surface Plasmon absorption at 418-420 nm), Energy Dispersive Spectroscopy
(EDX), Scanning Electron Microscopy (SEM) and X- ray diffraction (XRD). The cytotoxic effect of silver nanoparticles on acute myeloid leukemia cell line (THP – 1 cell line) was studied in vitro.
The activity of chemotherapeutic drugs Cyclophosphamide and Busulfan was enhanced in presence of silver nanoparticles whereas Mercaptopurine was found to be more effective when used individually against the THP- 1 cell line.
KEYWORDS:
Silver nanoparticles, Anti-cancerous property, Cyclophosphamide, Mercaptopurine, Busulfan
INTRODUCTION:
Nanotechnology is a promising arena and has exposed a panorama of applications in industrial, mechanical and medical field. In general nanoparticles are defined as particles with size < 100nm.
Various methods are used for the synthesis of metallic silver nanoparticles like chemical 1, electrochemical 2, laser ablation 5, photochemical 4 and biological, γ-radiation 3. Among all metallic nanoparticles, silver (Ag) nanoparticles are used in diverse fields because of their unique physical, chemical as well as their anti cancerous property
6
and antimicrobial
7
Silver nanoparticles are
generally produced by chemical methods
References: Science (MEDŽIAGOTYRA). Vol. 12, No. 4. 2006 2. Electrochemical method for the synthesis of silver nanoparticles, J Nanopart Res (2009) 11:1193–1200 Physics and Chemistry 79(2010)1240–1246 4. nanomaterials, Vol. 2011,265287,7 5. of Nanomaterials, vol. 2010, Article ID 142897, 7 pages, 2010. doi:10.1155/2010/142897 6. Angshuman Pal, Sunil Shah and Surekha Devi. Microwave-assisted synthesis of silver nanoparticles using ethanol as a reducing agent,Materials Chemistry and Physics 114 (2009) Combination with Ionic Liquids, Bull. Korean Chem. Soc. 2011, Vol. 32, No. 5 1583 11. S.Navaladian, B. Viswanathan, R. P. Viswanath, T. K. Varadarajan.Thermal decomposition as route for silver nanoparticles, Nanoscale Res Lett (2007) 2:44–48 XXXVIII, No. 1, 2008 13. Chem., 2007,9, 852-858 17.