Materials and Methods: The Mark-Recapture method was used to gather data for each pond. Groups of five – six students collected tadpoles from each pond. Three students collected tadpoles using a kick-net. A second group of students then measured and marked each tadpole. Marking was done by cutting a …show more content…
small notch out of the dorsal fin of the tail. The total length (mm) of each tadpole was measured from the tip of the snout to the base of the tail; this data was recorded using a field portable computer system. The first census consisted of two separate collections and combination of data from each collection was used. The second census was conducted using the same methods as the first census, however the number of marked tadpoles was simply recorded, no further marking occurred.
Results: From the data of each census I was able to calculate population size (N, individuals per area), Dispersion, and Density of tadpoles for each pond( Table 1).
Pond 1 had the highest population density of tadpoles, followed by Pond 4. A 95% confidence interval data from each pond overlapped, indicating that there was no significant statistical difference in population from pond to pond. Dispersion of the tadpoles was calculated, resulting in a value >1, placing the tadpole populations into a clumped dispersion pattern. The Chi-Square test was used to test the ratio of dispersion against a pre-determined critical value. The Chi-Square test showed a value > 5.99 (critical value), confirming that the conclusion about dispersion holds true that the tadpoles are of clumped dispersion. Pond 1 was determined to be the most densely populated pond, showing the hypothesis to be incorrect. Pond 2 showed to have the largest average body length, but also the lowest population density (Table
2).
Discussion: Although the results of this experiment did not support our original hypothesis, it does lead to some interesting interpretation. Pond 4 was initially hypothesized to have the most tadpoles due to its proximity to the wooded area, cattail cover, and direct sunlight received. The results showed Pond 1 to be most densely populated pond with Pond 2 having the largest average length tadpoles. This could be due to many factors such as varying drainage of each pond or food sources for tadpoles varying by location. Errors in collection could include collection and measuring techniques of each group of students. This could account for the low initial collection in Pond 2.