The effect of different light wavelengths was tested in the following experiment. The rate of photosynthesis can be affected depending on the light treatment it receives. With the aid of DPIP acting as NADPH in photosynthesis, it was possible to observe the reaction as it took place. Samples were created with dark, 24 cm, 30 cm, and 49 cm light treatments. The absorbance was measured in five-minute intervals for each sample and recorded in an appropriate table. When analyzing the data, it was observed that the 30 cm and 49 cm samples experienced a dramatic absorbance value decrease after the first five minutes, thus showing that the farther the distance from the light, the slower the reaction will take place.
The Effect of Light Wavelength
This experiment was conducted to observe the effect different light colors had on photosynthesis. Red, blue, green, white, and no light were the sample subjects. Spinach leaf disks were collected and placed into a beaker, with ten leaves under each light specified wavelength, with bio-carbonate to release all the excess gases. When the gases were released the disks would float. The rate at which this occurred could be used to determine the rate at which photosynthesis would occur.
The floating disks with each light were recorded in five-minute intervals.
The results of the floating disks according to different wavelengths showed that blue and white were the only wavelengths to experience the release of excess gases within a thirty-minute time period. The white light experienced the most success by concluding the thirty minutes with eight floating disks out of ten.
The Activity of Photosynthetic Pigments
Silica gel chromatography can be used to determine what light wavelengths are used for photosynthesis. In this experiment, chlorophyll a, chlorophyll b, carotene, and xanthophyll were tested. The distance moved by molecule and the distance moved by the solvent is used to