Unknown Bacteria Lab Report
Unknown Bacterium #22
Purpose
The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol
•Safrin •Glass Slides
•Microscope •Unknown bacteria in test tube
Procedure
On the first day, one plate was streaked qualitatively and left it in the 25 degrees celsius incubator over five days and attempted to gram stain from the test tube. On the second day, took out my plate from the incubator and examined the plate for bacteria. Then, three more plates where streaked qualitatively and placed …show more content…
This was compleated by growing the bacteria on a petri dish with agar, gram staining, then examning under a microscope. The bacteria identified was M. Roseus and B. Megaterium. The identity of these bacteria was mostly determined by the positve gram stain results and the shape they where under the microscope. Both of these bacteria where identified as gram positive (as shown in the table previously). This was determined beause of the nonexistant fatty layer in the cell’s cell wall that trapped the purple crystal violate. The second big thing that identified these bacteria was the shape you could see under the microscope. While looking under the oil immersion lense, the B. Megaterium was shaped as a rod, and the M. Roseus was shaped as a circle. The diference between these two was, the rod was a much longer and skinnier shape compared to the circle whitch was smaller and more round than the rod was. The error in this experiment was that during gramstaining the second bacteria. I was able to identify three different bacteria. One was small purple circles (the M. Roseus), another was purple rods (B. Megaterium), and the last one was small pink circles that was not identified. In this lab, one of the problems that was encountered was the bacterias would not grow. Although this was probably because failing to cool the loop while streakig, this was still one one of the main problems encountered. Another issue that was
Purpose
The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol
•Safrin •Glass Slides
•Microscope •Unknown bacteria in test tube
Procedure
On the first day, one plate was streaked qualitatively and left it in the 25 degrees celsius incubator over five days and attempted to gram stain from the test tube. On the second day, took out my plate from the incubator and examined the plate for bacteria. Then, three more plates where streaked qualitatively and placed …show more content…
This was compleated by growing the bacteria on a petri dish with agar, gram staining, then examning under a microscope. The bacteria identified was M. Roseus and B. Megaterium. The identity of these bacteria was mostly determined by the positve gram stain results and the shape they where under the microscope. Both of these bacteria where identified as gram positive (as shown in the table previously). This was determined beause of the nonexistant fatty layer in the cell’s cell wall that trapped the purple crystal violate. The second big thing that identified these bacteria was the shape you could see under the microscope. While looking under the oil immersion lense, the B. Megaterium was shaped as a rod, and the M. Roseus was shaped as a circle. The diference between these two was, the rod was a much longer and skinnier shape compared to the circle whitch was smaller and more round than the rod was. The error in this experiment was that during gramstaining the second bacteria. I was able to identify three different bacteria. One was small purple circles (the M. Roseus), another was purple rods (B. Megaterium), and the last one was small pink circles that was not identified. In this lab, one of the problems that was encountered was the bacterias would not grow. Although this was probably because failing to cool the loop while streakig, this was still one one of the main problems encountered. Another issue that was