Urine Dipstick Lab Report
Student number: 1701974
Unit code: BHS004-1
Assessment number: 2/5
Word count:
You were given five samples of patients’ urine and some Uristix® dipstick. Describe how you used the dipsticks to determine if urine sample contained amounts of protein and / or glucose.
To test the given samples for glucose or protein, dipsticks were immersed into each of the urine samples for 60 seconds. The colour change was compared with the colour chart on the Uristix® bottle.
The detection of glucose in urine is based on the presence of two enzymes, glucose oxidase and peroxidase. The first enzyme, glucose oxidase, catalyses the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose. The second enzyme, peroxidase, catalyses the …show more content…
It was the only tube that contained just BSA, without any diluent. The second tube was prepared by adding a 250 μl BSA and 250 μl of 0.9 % Saline (diluent). Following two tubes were prepared with the same amount of diluent. However, the volume of BSA added was different. Therefore, 250 μl from the tube 2 was pipetted to the tube 3, then the same amount was pipetted from tube 3 to tube 4. The fifth tube contained 800 μl of diluent and 200 μl of liquid transferred from tube 4. Lastly, tube 6 was a ‘blank tube’, containing only 250 μl of diluent. Based on the known volume of diluent and BSA, the final BSA (protein) concentrations of tubes were as following: 1000 μl in tube 1; 500 μl in tube 2; 250 μl in tube 3, 125 μl in tube 4; 25 μl in tube 5 and the final tube comprised of 200 μl of …show more content…
and Catto, J.W.F. (2008) 'Haematuria', Surgery, 26(4), pp. 150-153.
Flores, R. (1978) 'A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue', Analytical Biochemistry, 88(2), pp. 605-611.
Meurman, J.H., Rytömaa, I., Kari, K., Laakso, T. and Murtomaa, H. (1987) 'Salivary pH and glucose after consuming various beverages, including sugar-containing drinks', Caries research, 21(4), pp. 353-359.
Miller, N.L. and Lingeman, J.E. (2007) 'Management of kidney stones', British Medical Journal, 334(7591), pp. 468.
Mulryan, C. (2011) 'Urine testing through the use of dipstick analysis', British Journal of Healthcare Assistants, 5(5), pp. 234-239.
Olson, Bradley J S C and Markwell, J. (2007) 'Assays for determination of protein concentration', Current protocols in protein science, Chapter 3, pp. Unit 3.4.
P Trinder (1969) 'Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen', Journal of clinical pathology, 22(2), pp. 158-161.
Parmar, M.S. (2004) 'Kidney stones', BMJ, 328(7453), pp. 1420-1424.
Walker, J.M. (1994) 'The bicinchoninic acid (BCA) assay for protein quantitation', Methods in molecular biology (Clifton, N.J.), 32, pp.
Unit code: BHS004-1
Assessment number: 2/5
Word count:
You were given five samples of patients’ urine and some Uristix® dipstick. Describe how you used the dipsticks to determine if urine sample contained amounts of protein and / or glucose.
To test the given samples for glucose or protein, dipsticks were immersed into each of the urine samples for 60 seconds. The colour change was compared with the colour chart on the Uristix® bottle.
The detection of glucose in urine is based on the presence of two enzymes, glucose oxidase and peroxidase. The first enzyme, glucose oxidase, catalyses the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose. The second enzyme, peroxidase, catalyses the …show more content…
It was the only tube that contained just BSA, without any diluent. The second tube was prepared by adding a 250 μl BSA and 250 μl of 0.9 % Saline (diluent). Following two tubes were prepared with the same amount of diluent. However, the volume of BSA added was different. Therefore, 250 μl from the tube 2 was pipetted to the tube 3, then the same amount was pipetted from tube 3 to tube 4. The fifth tube contained 800 μl of diluent and 200 μl of liquid transferred from tube 4. Lastly, tube 6 was a ‘blank tube’, containing only 250 μl of diluent. Based on the known volume of diluent and BSA, the final BSA (protein) concentrations of tubes were as following: 1000 μl in tube 1; 500 μl in tube 2; 250 μl in tube 3, 125 μl in tube 4; 25 μl in tube 5 and the final tube comprised of 200 μl of …show more content…
and Catto, J.W.F. (2008) 'Haematuria', Surgery, 26(4), pp. 150-153.
Flores, R. (1978) 'A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue', Analytical Biochemistry, 88(2), pp. 605-611.
Meurman, J.H., Rytömaa, I., Kari, K., Laakso, T. and Murtomaa, H. (1987) 'Salivary pH and glucose after consuming various beverages, including sugar-containing drinks', Caries research, 21(4), pp. 353-359.
Miller, N.L. and Lingeman, J.E. (2007) 'Management of kidney stones', British Medical Journal, 334(7591), pp. 468.
Mulryan, C. (2011) 'Urine testing through the use of dipstick analysis', British Journal of Healthcare Assistants, 5(5), pp. 234-239.
Olson, Bradley J S C and Markwell, J. (2007) 'Assays for determination of protein concentration', Current protocols in protein science, Chapter 3, pp. Unit 3.4.
P Trinder (1969) 'Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen', Journal of clinical pathology, 22(2), pp. 158-161.
Parmar, M.S. (2004) 'Kidney stones', BMJ, 328(7453), pp. 1420-1424.
Walker, J.M. (1994) 'The bicinchoninic acid (BCA) assay for protein quantitation', Methods in molecular biology (Clifton, N.J.), 32, pp.