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Abstract For our most recent experiment we tested the effects of immersing water fleas in caffeine and alcohol to see the effects on their heart rate. Before the experiment we hypothesized that if you place the water fleas in alcohol then their heart rate will slow down, and if you place them in caffeine then their heart rate will raise. For this experiment we needed water fleas, tap water, two pipettes, a microscope, a stopwatch, two glass micro slides, caffeine (concentrations: 1.0, 1.5, 2), alcohol (concentrations: 2.0, 4.0, 6.0). We then used the pipette to place one water flea into a concentration of water as the control. Once the control was established we proceeded to add different concentrations of caffeine and alcohol to the slide. We used a stopwatch to observe the heart rate and found that at first the rate decreased and then increased as we tested higher concentrations of caffeine. We then found that as we added alcohol the water fleas heart rate increased and then decreased as we tested higher concentrations. Materials and Methods In this experiment we used a variety of different materials. The first was the microscope. This was used to view the water flea at a magnification of 100x. Without this we could not have seen the heart rate of the flea. Next, we obtained two glass micro slide. These were used to hold the Daphnia as we continued with the experiment. Before being able to get the Daphnia onto the slides we had to draw them up with two pipettes. A stopwatch was then used to determine the control, as well as variable 's heart rates. Lastly, let 's not forget the star of the show, the Daphnia itself. Without these little critters the whole experiment would be a bust. To obtain the control of this experiment we prepared a slide with a single drop of water and a single Daphnia. We then counted the Daphnia 's heart rate for one minute.
Cited: "Effects of Caffeine on the Heart Rate of the Human Body." LIVESTRONG.COM. N.p., n.d. Web. 23 Oct. 2012. . The Independent. Independent Digital News and Media, n.d. Web. 23 Oct. 2012. .