Jennie Halula
1/23/2015
WCCC/Spring 15’
A&P2/Laboratory
Mr. Sisak
Introduction
The purpose of this experiment is to run the following blood tests on an individual (myself) and to record results.
1. RBC count (Normal Value: Females:4.8 million, Males:5.4 million per mm of blood)
2. WBC count (Normal Value: 4,500-11,000 per mm of blood)
3. WBC differential
a. Normal Value: Neutrophils: 50-70%
b. Normal Value: Eosinophils: 1-4%
c. Normal Value: Basophils: 0.5-1%
d. Normal Value: Lymphocytes: 20-40%
e. Normal Value: Monocytes: 2-7%
4. Hematocrit (Normal Value: Females: 38%-46%, Males: 42%-56%)
5. Hemoglobin (Normal Value: Females: 12–16 g/dL, Males: 14–17.4 g/dL)
6. Calculate MCHC
Red Blood Cell Count:
Red blood …show more content…
Calculate the percentage of the various types of leukocytes and place in date table.
Hematocrit (Packed Cell Volume) Method
1. Procedure
a. Obtain 2 heparinized capillary tubes, a sharp, an alcohol pad, a bandage and a clay pad.
b. Using proper sterile technique, perform a finger stick.
c. Fill 2 heparinized capillary tubes with blood (at least 2/3 full).
d. Seal one end of the capillary tube by pressing the non-bloody end into the clay pad and capillary bottom so that the clay is firmly embedded.
e. Place the sealed capillary tubes into the numbered slots of the microcentrifuge with the clay pointing to the outside. (Make sure that the centrifuge is balanced).
f. Screw the cover plate on the centrifuge, close the lid.
g. Turn on the centrifuge and spin for 3 minutes.
h. At the end of the centrifugation, the hematocrit is read by utilizing a hematocrit reader card. Place the top of the clay pack at the 0 line and the top of the plasma at 100 line. The hematocrit value is obtained by reading the value of the line at the top of the red blood cell pack.
i. Dispose of hematocrit tubes in the sharps container.
j. Record results in data table.
Hemoglobin
1. Procedure
a. Remove one square of paper from the Tallquist