Why When Touching The Surface Of The Paper To Be Used For Amino-Acid Chromatography
Questions
1. Why must you use lead pencil, instead of a pen, to mark your chromatography paper?
A lead pencil should be used instead of a pen because the ink of the pen would dissolve in the eluting solution and separate. The components of the ink could mix with the other ink/ dyes that would be tested on the same chromatogram. Thus, a lead pencil should be used. 2. Why should you avoid touching the surface of the paper to be used for amino-acid chromatography?
We should avoid touching the surface of the chromatogram because the oils on our skin can affect the polarities of the substances. As a result, this would affect eh data gathered from the experiment and the calculation made using that data. 3. Where is the kitchen, or in the …show more content…
For example, meats has protein. Protein is made of polypeptide chains, which is made of amino acids. Furthermore, in the cells in the body has protein. Thus, there is amino acids in our body. 4. If two different substances have the same or nearly the same Rf values, it is difficult to separate (resolve) these materials in a mixture. How could this experiment be changed to separate such a mixture? This experiment can be changed by adding a color indicator like markers or inks to show the separation of the materials in a mixture. 5. Give two or three practical examples where chromatography would be a valuable analytical tool.
One example where chromatography would be valuable is synthesizing the cure for a poison or creating an antivenom for a snake venom. Another practical use of chromatography is for criminal investigations, where there are murder scene and the agent attempts to find the cause of death. 6. The Rf of a spot contains information regarding the attraction of the substance being chromatographed to the paper and the eluting solution. Using your data:
a) Which amino acid has the strongest attraction to the paper? (Explain your answer.)
The leucine amino acid has the strongest attraction to the paper because it has the highest representation fraction of
1. Why must you use lead pencil, instead of a pen, to mark your chromatography paper?
A lead pencil should be used instead of a pen because the ink of the pen would dissolve in the eluting solution and separate. The components of the ink could mix with the other ink/ dyes that would be tested on the same chromatogram. Thus, a lead pencil should be used. 2. Why should you avoid touching the surface of the paper to be used for amino-acid chromatography?
We should avoid touching the surface of the chromatogram because the oils on our skin can affect the polarities of the substances. As a result, this would affect eh data gathered from the experiment and the calculation made using that data. 3. Where is the kitchen, or in the …show more content…
For example, meats has protein. Protein is made of polypeptide chains, which is made of amino acids. Furthermore, in the cells in the body has protein. Thus, there is amino acids in our body. 4. If two different substances have the same or nearly the same Rf values, it is difficult to separate (resolve) these materials in a mixture. How could this experiment be changed to separate such a mixture? This experiment can be changed by adding a color indicator like markers or inks to show the separation of the materials in a mixture. 5. Give two or three practical examples where chromatography would be a valuable analytical tool.
One example where chromatography would be valuable is synthesizing the cure for a poison or creating an antivenom for a snake venom. Another practical use of chromatography is for criminal investigations, where there are murder scene and the agent attempts to find the cause of death. 6. The Rf of a spot contains information regarding the attraction of the substance being chromatographed to the paper and the eluting solution. Using your data:
a) Which amino acid has the strongest attraction to the paper? (Explain your answer.)
The leucine amino acid has the strongest attraction to the paper because it has the highest representation fraction of