AP Biology
Lab Report Number One
Mrs. Irvine
Introduction: Because all molecules have kinetic energy and are constantly in motion cells go through a process called diffusion. Diffusion is the movement if molecules from an area of higher concentration to and area of lower concentration. This process with continue to occur until an equilibrium is reached. Osmosis is a different and unique kind of diffusion. Osmosis is the diffusion of water through a permeable membrane. The phrase “permeable membrane” means that the membrane will only allow specific molecules through such a water or oxygen. In Osmosis water will travel from an area of higher water potential or an area of lower water potential.
Hypothesis:
I think that in this lab, osmosis and diffusion will occur between the solutions of different concentrations until a equilibrium is reached and there is no movement of water.
Materials:
EXERCISE 1A: Diffusion
The materials include a 30-cm piece of 2.5-cm dialysis tubing, 15-mL of the 15% glucose/1% starch solution, 250-mL beaker, distilled water, 4-mL Lugol’s solution, and string. EXERCISE 1B: Osmosis
The materials used include 25-mL of these solutions: distilled water, 0.2M sucrose, 0.4M sucrose, 0.6M sucrose, 0.8M sucrose, and 1.0M sucrose, scissors, string, a balance, six 250-mL cups, and six 30cm strips of dialysis tubing. EXERCISE 1C: Water Potential
The materials that were used included 50mL of distilled water, 0.2M sucrose, 0.4M sucrose, 0.6M sucrose, 0.8M sucrose, and 1.0M sucrose, six 250mL cups with lids, 4 potato cores for each cup, a balance, and paper towel. EXERCISE 1D: Calculation of Water Potential from Experimental Data
This exercise required a calculator and a pencil.
Procedure:
EXERCISE 1A: Soak the dialysis tubing in water before you start the experiment. Tie off one end of the tubing to form a bag like structure. Through the open end of the bad, place the