1. What is the molecular weight of Na+? 22.99 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚ 100 and 200 4. Which materials diffused from the left beaker to the right beaker Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin‚ because it is too large to diffuse Activity 2: Simulating Dialysis 1. What happens to the urea concentration in the left beaker (the patient)? Its concentration gradient
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Hypothesis – Activity 2: Simulating Facilitated Diffusion “I hypothesize that a higher concentration gradient would result in a higher rate of glucose transport” Materials and Methods: Activity #1 Materials: * 2 Beakers * 4 MWCO Membranes * 20‚ 50‚ 100‚ 200 * 9.00 mM of each solute: * NaCl‚ Urea‚ Albumin‚ Glucose * Deionized water Methods: * The solutes NaCl‚ Urea‚ Albumin‚ and Glucose each in the amount of 9.00 mM were used respectively. * The first step
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concentration. 3. Which of the following dialysis membranes has the largest pore size? You correctly answered: d. 200 MWCO 4. Avogadro’s number is a constant for the number of You correctly answered: b. molecules. 06/17/14 page 1 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No‚ not at all. Predict Question 2: Recall that glucose is a monosaccharide‚ albumin
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Size of material and concentration 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? The molecules were too large to pass through. This is what I predicted on my Hypothesis. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? The glucose diffused through the
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the Pre-lab Quiz. 09/20/11 page 1 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No‚ not at all. Predict Question 2: Recall that glucose is a monosaccharide‚ albumin is a protein with 607 amino acids‚ and the average molecular weight of a single amino acid is 135 g/mole. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer :
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diffusion are the MWCO membrane and the solute concentration. Increasing the membrane size and solute concentration will also increase the average diffusion rate. Decreasing the membrane size and solute concentration will reduce diffusion rates and can even prevent all diffusion. 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: The urea was not able to diffuse through the 20 MWCO membrane because
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able to diffuse into the right beaker. Using distilled water in the right beaker and either the 100 MWCO or 200 MWCO membrane will remove urea from the left beaker and leave albumin If the left beaker contains NaCl‚ urea‚ and albumin‚ you can selectively remove urea by dispensing a concentration of NaCl into the right beaker equivalent to that in the left beaker and by using the 100 or 200 MWCO membrane. Albumin is too large to diffuse and there will be no net diffusion of NaCl. However‚ urea
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kinetic energy of the molecules in motion. 2. In diffusion‚ molecules move You correctly answered: a. from high concentration to low concentration. 3. Which of the following dialysis membranes has the largest pore size? You correctly answered: d. 200 MWCO 4. Avogadro’s number is a constant for the number of You correctly answered: b. molecules. 09 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. The effect of increasing the concentration of sodium chloride
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Simple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? According to your results‚ which solute had the highest molecular weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute.
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able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Since the chemical formula for urea is (NH2)2CO and its molecular weight is about 60. It will not diffuse through the 20 MWCO membrane because it does not ionize. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Glucose will diffuse through the 200 MWCO membrane because its MW is 180
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