DATE PERFORMED: JANUARY 6‚ 2011 SPECTROPHOTOMETRIC DETERMINATION OF THE EQUILIBRIUM CONSTANT OF A REACTION ABSTRACT The objective of the experiment was to determine the equilibrium constant of the reaction forming ferric thiocyanate through the use of Spectrophotometry. For the calibration‚ five standard solutions were prepared‚ then their respective absorbance values that were obtained through the use of the spectrophotometer‚ were plotted versus the concentration of the analyte so that a calibration
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Weekly iLab iLab: Exploring the Microbiology Lab Section 1 Lab Safety 1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet‚ Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid loose
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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absorbance of salt‚ dead yeast cells‚ contamination‚ plate overgrowth‚ and the yeast cells drying from being under the vent in the incubator. In the future‚ we can do this experiment more effectively by having more than three weeks to perform the experiment as well as letting the yeast grow for more than twenty-four hours. As a result of having a longer amount of time to do the study and letting the yeast grow for longer than twenty four hours‚ we would get better results; yeast multiplies and divides
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Since the Grignard reagent can easily react with water‚ all glassware including the 25 ml round bottom flask‚ magnetic stir bar‚ 3 and 5 ml conical vial‚ 50 mL Erlenmeyer flask‚ claisen adapter‚ drying tube and 5 glass pasteur pipets were first added to a 250mL beaker and placed in the oven for 30 minutes. After the completion of the thirty minutes‚ 0.150 g of shiny magnesium turnings and a stir bar was first added to the round bottom flask and the claisen adapter along with the drying tube packed
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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Michele Hindmarsh mhindma@my.wgu.edu Student ID# 000383032 MLT1 – Experiment 5; Task 6 Differential Staining Heidi Atkinson‚ MS Lab Experiment #5-Differential Staining Through the process of differential staining‚ there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan
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In this lab‚ I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than
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Density (Linearized plot) TA: Blue Rex rex Group Members: Billy and Mandy Tuesday; 1200-1350 Abstract: In this lab the density of hand-made clay balls were calculated to understand how scientists model physical effects and to understand logarithmic plots. The hand-made balls ranged from diameters of 2cm to 6cm and were measured with vernier calipers by each member of the group. A total of 6 independent measures of each diameter were taken to establish uncertainty. The clay balls were then
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Lab Experiment 8: Oxidation Puzzle Post Lab Report for 2-ethyl-1‚3-hexandiol Calculations Theoretical yield: 1.857g Product Yield: 1.055g ----> Percent yield = (1.055g/1.87g) x 100% = 56.41% Starting amount of diol: 1.184g ----> Percent Yield = (1.184g/1.87g) x 100% = 63.32% Spectroscopy O-H (Stretch‚ H-bonded) C-H (Stretch) C-H (2720-2820 cm-1) Carbonyl C-O (Stretch) Product wavelength cm-1 3422 Strong‚ Broad 2877‚2936‚2964 Strong‚ Medium None Present 1705 Strong
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