Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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not a good resonance structure? 6. How many sigma and pi bonds are in cyanogen :N TripleBOND C – C TRIPLEBOND N: 3 sigma bonds and 4 pi bonds 7. What is the only allowed value of l if n = 3 and ml = -2? l=2 8. Which bond angle in acetone has a value of 120 degrees? Lewis structure C-C-C is ~ 120 degrees Trigonal planar ‚ ~120 degree
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Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas putida A Project done under the guidance of Dr. K. Bharathi Department of Biotechnology. Submitted to the faculty Of Department of Biotechnology National Institute Of Technology‚ Warangal (A.P) Submitted By Febin P. Nalpady‚ Anzal Rahman‚ Shruti Sharma‚ Sindhuja Nandiraju‚ Giraboina
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Distillation Purpose: To conduct the distillation process‚ simple or fractional. Procedure: 1. Add 5ml of 20% ethanol: water mix to the distillation flask. 2. Add 23 stir bar 3. Make sure that the thermometer bulb is just below the Claisen Head‚ carefully. Be very gentle with the thermometers and take care not to break them. 4. Label 3 vials‚ 1‚2‚ and 3. 5. Place receiving vial 1 on ice to collect the distillate 6. Start the distillation by piling up hot sand around the distillation flask with
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sulphide etc and hence smells bad. Acetylene can be liqiufied at 0°C under 48 Torrs of pressure. The melting point of Acetylene is -81.8°C and boiling point -83.4°C. It is slightly soluble in water but highly soluble in organic solvents like alcohol and acetone. Acetylene under suitable conditions decomposes with evolution of a lot of heat and sudden increase in volume‚ which causes explosion. Hence liquid acetylene is highly explosive. ------------------------------------------------- Uses Acetylene
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Leo Tran IB Chemistry SL II R5 Ms. Crane January 4‚ 2012 Restoring Balance LAB Pre-Lab Questions 1. The solubility of iodine increases because the position of equilibrium has shifted towards the triiodide side of the reaction. Addition of the reactants causes an increase in the product which is directly related to the solubility of iodine in water in this case. 2. Ammonium chloride will be more soluble in hot water as in an endothermic reaction‚ increasing the temperature (which
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ABSTRACT: Medicinal plants are potential sources of new drugs to improve the treatment of diseases whose treatment is associated to anti-oxidative agents. In the current study‚ we carried out a comparative analysis of the antioxidant activities of extracts of the selected leaves of 6 common medicinal plants. Total Ascorbic Acid Content‚ Total Carotenoid Content‚ Antioxidant potential using FRAP assay and Total Phenol Content was quantitatively estimated from leaves of Rauvolfia serpentina‚ Cymbopogon
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Analysis of Lipid through Two-Dimensional Thin Layer Chromatography Myca Pua‚ Ramon Ramos‚ Amanda Redilas‚ Kimleigh Reyes‚ Nathaniel Sim and Clara Tamondong Group 9 2F Medical Technology Biochemistry Laboratory ABSTRACT Lipids constitute a group of naturally occurring molecules that include fats‚ waxes‚ sterols‚ fat-soluble vitamins such as vitamins A‚ D‚ E‚ and K‚ monoglycerides‚ diglycerides‚ triglycerides‚ phospholipids‚ and others. In this experiment lipid was extracted from the egg yolk
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Exp.11) Identification of unknown ketones. Introduction: Given five samples of a known ketone derivative‚ the purpose of this experiment is to identify which unknown ketone derivative corresponds to the five known samples. In other words‚ using specific methods of compound detection‚ it is possible to match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment‚ identification of the unknown ketone is accomplished through thin
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