GENERAL BIOCHEMISTRY Problem Set No. 1 Answered by: Andrea Rose A. Fajardo of BSFT 2-1N 1. Calculate the pH of a dilute solution that contains a molar ratio of potassium acetate to acetic acid (pKa=4.76) of: a. 2:1 pH = 4.76 + log[2]/[1] = 4.76 + 0.3010 = 5.06 b. 1:4 pH = 4.76 + log[1]/[4] = 4.76 + (-0.6021) = 4.16 c. 6:5 pH = 4.76 + log[6]/[5] = 4.76 + 0.0792
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NaOH to raise pH by 2 units (mL) | | | Volume of 0.5 M HCl to lower pH by 2 units (mL) | | | Volume of 0.5 M NaOH at equivalence point (mL) | | | Data Analysis 1. Write reaction equations to explain how your acetic acid-acetate buffer reacts with an acid and reacts with a base. 2. Buffer capacity has a rather loose definition‚ yet it is an important property of buffers. A commonly seen definition of buffer capacity is: “The amount of H+ or OH– that can be neutralized before the
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Investigating the effect of pH on amylase activity This practical allows you to: * discover how pH affects the rate of an enzyme controlled reaction * evaluate the experimental procedure Procedure SAFETY: Follow your teacher’s instructions for handling the solutions. Wear eye protection when handling the iodine solution. Investigation * Place single drops of iodine solution in rows on the tile. * Label a test tube with the pH to be tested. * Use the syringe to place
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DETERMINING THE EQUIVALENT MASS AND DISSOCIATION CONSTANT OF AN UNKNOWN WEAK ACID BY TITRIMETRY INTRODUCTION Acids are substances that donate hydrogen ions and bases are substances that accept hydrogen ions. Acids and bases react with each other by transferring hydrogen ions. One way to distinguish an acid is by its equivalent mass‚ which is the number of grams of the acid needed to transfer one mole of hydrogen ion to a base. For a monoprotic acid‚ which only transfers one hydrogen ion‚ its
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centrifugal speed brings about the sedimentation of first the cytoplasmic large and small granules. RNA was obtained from the cytoplasmic fractions. After centrifugation‚ the supernatant was collected while the residue was discarded. Glacial acetic acid was added in the supernatant until it became slightly acidic. The obtained solution was again centrifuged and filtered through the cheesecloth. Centrifugation and filtration was performed many time until the supernatant became clear. The supernatant
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discarded soln and washed ppt and centrifuged again. 3) I added 2mL of H2O‚ put the test tube in the hot water bath and stirred for 3 minute with a stirring rod. 4) I then decanted the solution into another test tube. 5) I added one drop of 6M acetic acid and 3 drops of 1M K2CrO4‚ which formed a positive confirmation where yellow ppt was formed‚therefore it contained Pb+2 Identifying Hg2+2: 1) I took the white ppt formed formed from the first test and added 10 drops of 6M NH3. 2) I stirred the contents
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148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make 1x TAE 20 L‚ add 400 ml 50X buffer into 19.6 L ddH2O. 4. SDS-PAGE Gel Solutions Vol (L) Tris (g) HCl (ml) 10% SDS (ml) 4x Lower gel buffer 1.5 M Tris-Cl‚ pH 8.8‚ 0.4% SDS 2 363.3 50-60 80 ml 4x Upper gel buffer
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Ale_____ Ram___ 30 de noviembre de 2012 _______________ BIOL 3503 Ecología Laboratory #1: Communities and Biomes 1. List the fish and invertebrates you selected after the nitrogen cycling process. I personally selected a maroon clownfish with two anemones. I also chose 5 sergeant majors‚ 2 bleenies and a yellow tang. 2. What changes did you make to your reef tank during the 12 weeks and why did you make them? After I selected my fish‚ I noticed that the tank
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Q1. Why should a four place analytical balance not be used in weighing a sample if the manual requests only one decimal place accuracy? Ans. We use the analytical balance where we need to weigh the small amount which needs a high degree of accuracy. Whereas‚ the manual requests only one decimal place accuracy which is not that much important and the time is consumed more to use the analytical balance with four decimal places. Q2. How is the pH meter calibrated? Ans. Firstly‚ The pH meter is calibrated
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Preparation of buffer solutions 1. Activation buffer (Mixed Phosphate Buffer‚ pH 5.5) Solution 1: An accurately weighed quantity of 1.61 g of potassium dihydrogen phosphate was dissolved in sufficient deionized water to produce 100 mL of solution. Solution 2: An accurately weighed quantity of 35.81 g of disodium hydrogen phosphate was dissolved in sufficient volume of deionized water to produce 100 mL. Accurately measured volume of 96.4 mL of solution 1 was mixed with 3.6 mL of solution 2 to get
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