The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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When I received my slant culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram
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Lab Report: Acid and Base Indicator Lab The title of my lab report is “Acid and Base Indicator Lab” this lab is testing what household solutions area bases depending on what color they changed. You have to use the pH balance scale to determine Acid‚ Base‚ and Neutral. On this lab we used 7 different solutions to determine if it’s either base‚ acid‚ and neutral. These are ammonia‚ vinegar; drain cleaner‚ soft drink‚ baking soda‚ detergent‚ and lemon juice are the solutions that are going to be
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Acid Rain Lab Report Introduction: The problem was to see how crushed chalk and whole chalk weather differently. The independent variables are The temperature of the liquids The surface area of the chalk‚ crushed vs whole The dependant variable was the weathering of the chalk; this was calculated by measuring the foam reactant. Procedure: Part B- For the two partners recording data with the room temperature liquids‚ the materials where collected first. Two of the graduated
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Lab Report Acids and Alkalis: Chemistry of Neutralization and Salt Formation Introduction: An acid is a group of chemicals. Acids are positively charged ions‚ they are liquid and are solutions of pure compounds in water. If you want to know if something is an acid‚ you can test it by using litmus paper. Acids will turn litmus paper red‚ whilst alkalis will turn it blue. Alkalis are negatively charged ions and are usually solid. Aim: To find out how much of different acids is needed to neutralize
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Acid Rain Lab #1 Chem. 1 20 February 2016 Concordia University Texas Angelina Luna angelinaluna97@yahoo.com Chem 1- Sect C Acid Rain Lab #1 Professor Koeck Abstract In this experiment our main objective was to create three gaseous oxides CO₂‚ SO₂ and NO₂ by using data that was collected using a pH sensor which was connected to a laptop with LoggerPro and Vernier. We created the gases by bubbling each gas into the water to create the three acidic solutions we were looking for. Once that
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effects that acid rain has or does not have on seed growth and germination. The observation gathered from this experiment was that regular rain water that has a neutral pH is a better solution for seed growth. The seeds that were watered with the well water prospered more than the ones grown in the lemon juice solution. The results from this experiment were a little confusing‚ because in the first trial the seeds watered with well water germinated more than the seeds watered with the acid rain solution
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well it started to lift the lid off the dish. The results from the Acid Rain (50% vinegar solution) did not allow any radish seeds to germinate. There were never signs of seed germination while observing the seeds over a three week period. In the petri dish labeled Acid Rain‚ 0 out of 10 seeds sprouted into plant growth as seen in Figure 5.2. The results from the effects of acid rain are compared on Figures 5.1 and 5.2.
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