Investigating a factor that affects the rate of enzyme activity Enzymes speed up reactions. They have an area with a very particular shape called the ‘active site’. When the right molecule comes along (substrate molecule) it will fit perfectly into the active site and there will be a reaction. After the reaction the products then leave the active site. This process is often referred to as the lock and key theory as only one enzyme can carry out one type of reaction. The catalase enzyme speeds
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What affect does the concentration of sugar have on the rate of respiration of yeast? Apparatus: 1. Stopwatch with a resolution of 0.001 seconds 2. 4 test tubes 3. 200 ml of yeast in a beaker 4. 200 ml of glucose solution 5. 500 ml of limewater 6. 2 delivery tubes 7. 1 test tube rack 8. 1 water bath set to 37°C 9. 2 pipettes 10. 3 measuring cylinders with a resolution of 1ml‚ ranging from 0-30ml. 11. 200 ml of water Hypothesis: The higher the concentration
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concentrations of sucrose solution‚ and measure any changes in mass and length. Affecting Factors: There are a number of factors which can affect our results. We need to keep them all the same‚ and make sure that the only thing that changes is the concentration of the sucrose solution. These factors include: • Starting length of potato • Volume of Sucrose solution • Temperature of potato • Temperature of Sucrose solution • Time we left the potato in the Sucrose solution for
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Extraction of DNA from Calf or Hog Thymus/Isolation of Yeast RNA I. Abstract Nucleic acids may be divided into two groups RNA and DNA. DNA contains almost all the genetic information while RNA serves as the bridge between the DNA and proteins. Study of both DNA and RNA initially involves proper extraction/isolation. The storehouse of eukaryotic DNA is the nucleus (and in the mitochondria)‚ so experimentally‚ DNA is extracted from tissues that have a high nuclear to cytoplasmic mass ratio‚ such
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CHAPTER I INTRODUCTION Background of the study “Pain is only what you allow it to be”.- Cassandra Clare Pain is a subjective experience‚ and infants and children respond to pain with the behavioural reactions that depend upon the age and cognitive processes. Pain may occur as a result of procedures‚ surgery‚ illness or injury. During infancy‚ reflective behaviour is dominant. Between 3 and 10 months of age
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An investigation to find the water potential of potato and carrot tubers in a sucrose solution‚ of concentration 0.00 – 0.50Mol‚ over a 24 hour period Interpretation Written Communication C1 From our graph it can be seen that the concentration of sucrose solution is 0.18 M at 0% change in mass for the potato and 0.355 M at 0% change in mass for the carrot. I will use these values to find the solute potential by using the calibration graph. I will work out the water potential by using the
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mass decreased as sucrose concentration increased‚ therefore‚ relative osmotic concentration also decreased as sucrose concentration increased. However‚ the osmotic concentration of 0.2 M sucrose solution was relatively greater than that of 0.1 M sucrose solution. In sucrose concentration 0.5 M‚ the osmotic concentration decreased slightly from that of 0.4‚ but significantly from those of all other sucrose concentrations. The osmotic concentrations were greater than zero in sucrose solutions of 0
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oxygen‚ water‚ amino acids etc. to pass through but will not allow larger molecules like sucrose‚ starch‚ protein etc. through. A region of high concentration of water is either a very dilute solution of something like sucrose or pure water. In each case there is a lot of water: a high concentration of water. A region of low water concentration is the opposite of the above‚ i.e. a very high concentration of sucrose solution: a low water concentration. The water content of plants varies depending on
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Summary This purpose of this experiment was for students to do the colony count methods‚ estimating the viable cell number of commercial active dried yeasts (ADY). This experiment allowed the students to perform the plate count technique by serial dilution and two common methods‚ spread plate and pour plate to determine the colony forming unit (CFU) of yeasts A ten-fold dilution is used in this experiment‚ the sample is diluted until it reached the 10-9 dilution. Plating for spread plate started from
Free Agar plate Petri dish Yeast
of Energy Production During Yeast Fermentation Sarah Sulon Biology Lab 111L Dr. Murray October 25 2010 Abstract The experiment was conducted to determine the impact different sugar types have on yeast fermentation. It was hypothesized that glucose‚ sucrose and fructose would all produce energy through yeast fermentation‚ but that sucrose would have the greatest rate of energy production. The carbon dioxide production was tracked in the fermentation of yeast with solution of no sugar‚ glucose
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