Chemistry Beer’s Law & Colorimetry Submitted by Monika Rebalska Date Submitted: 3/7/2015 Date Performed: 3/5/2015 Lab Section: Chem-180 Course Instructor: Cynthia Spencer Purpose The purpose of this experiment was to construct a Beer’s Law Plot‚ determine the concentration of a sample using the Beer’s law plot‚
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Objective: The purpose of this lab is to demonstrate that there is a linear relationship between the number of molecules that can absorb light present in a solution and the amount of light absorbed by a solution. This lab should prove that Beer’s law and the equation A=a x b x c‚ is a linear relationship. Procedure: The only deviations in the lab procedure was that the stock solution was made before arrival to the lab with 0.570 g of KMnO4 in 0.500 L. The diluted solutions and the
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head: Beer’s Law And Calorimetry Beer’s Law And Calorimetry Adriane Bellard Ocean County College Beer’s Law is also referred to as the Beer- Lambert law or the Bouguer- Beer Law. The principle is based on an electromagnetic radiation that is passed through a sample‚ wavelength is detected by the sample. As a result strength of transmitted light is gradually reduced. The measurement of the reduced strength of radiation is supported by the spectrophotometer. Based on Beer’s Law
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Beer’s Law Lab Report Abstract: The Beer’s law lab was conducted to determine the optimal wavelength of Co(NO3)2·6H2O with the use of spectrometry. The results determined that the optimal wavelength to study the absorbance of this salt was 500nm. It also demonstrated how transmittance of light and absorbance of light are inversely proportional because absorbance is calculated by multiplying transmittance by a negative log. Introduction: When one is studying chemicals‚ there are many important
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Beer’s Law Data: Wavelength: 810 nm Table 1 Sample # mL of stock placed in the 100 mL flask Initial buret (mL) Final buret (mL) Actual mL used (mL) Calculated concentration (M) % T (%) Absorbance 1 1 1.19 2.19 1.00 .01 78.6 .105 2 2 .31 2.29 1.98 .0198 61.1 .214 3 3 2.29 5.31 3.02 .0302 46.2 .335 4 4 5.31 9.30 3.99 .0399 36.1 .442 5 5 9.30 14.31 5.01 .0501 27.6 .559 6 6 14.31 20.32 6.01 .0601
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Spectrophotometry/Beer’s Law Lecture Chemistry 114 Overview: Spectroscopy will be a tool that you will use as you continue in your chemistry‚ biology and physics courses. Already‚ you have used spectroscopy in this course and CHM 113. Earlier in the term‚ we used the spectrophotometer to monitor double stranded and single stranded DNA. We have also used it to measure concentration of chemicals. This lecture will discuss spectophotometry in more detail. Background: You will recall from
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FORMAT FOR LAB REPORT-General Chemistry A lab report is both a record of your qualitative and quantitative observations as well as an analysis of you laboratory work. It is an original; it is your creation. It is not a copy of something another person wrote. Trust yourself and be willing to learn from your mistakes. Lab reports are based on 100 points. The report will be graded on accurate reporting of information‚ proper use of the format‚ completeness and quality of analysis and conclusion
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determine the concentration and equilibrium constant for a reaction of colored product using absorption spectroscopy‚ to create a calibration curve for determining equilibrium concentrations by applying Beer’s law and to force the completion of a reaction by applying Le Châtelier’s principle. Procedure: A diluted solution was made from the stock of 0.0025 M Fe(NO3)3 by diluting 4.0 mL to exactly 100.00 mL‚ using serological pipet and volumetric flask. The concentration of the diluted solution was
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BEER’S LAW EXPERIMENT 3 Amanda Buchanan – September 20‚ 2015 Chemistry 1212 – Section 50 OBJECTIVES: The objectives of this experiment are to understand why and how spectroscopy is used to determine the components and concentrations of a solution‚ describe various types of spectroscopy‚ describe the visible and ultraviolet regions of the electromagnetic spectrum‚ define Beer’s law and define the relationship between absorbance and transmittance. Other learning objectives are to create a Beer’s
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SPECTROPHOTOMETRY Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength It involves with the use of a spectrophotometer. A spectrophotometer is a photometer that can measure intensity as a function of the light source wavelength. A spectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions‚ transparent or opaque solids‚ such as polished glass‚ or gases. Principles
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