Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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Science 8 Observing Onion Cells ***Do not write anywhere on this piece of paper!!! It will be used again this afternoon!!!***** Instructions. On a piece of paper‚ write the title (see above)‚ the date‚ your block‚ and your name. Then copy out the problem (see below). Problem. What structures can you observe in an onion cell when using a compound light microscope? Hypothesis. On your lab report‚ below your Problem‚ write a Hypothesis that will “answer” the problem. Start your
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Acids‚ Bases and Buffers Lab Acids‚ Bases and Buffers Lab Results: The experimental results for part one is as follows: Part One Data Table | Initial pH | Final pH | Test Tube A | 6 | 1 | Test Tube B | 4 | 4 | Test Tube C | 4 | ----- | Test Tube D | 4 | 4 | Test Tube E | 6 | 11 | The experimental results for part two is as follows: Part Two Data Table | Before CO2 was Added | After CO2 was Added | Colour | Blue/green | Light green/yellow | pH Level | 8.0pH | 5.0pH |
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I. Introduction A buffer system is a mixture of a weak acid or a weak base and its salt (conjugate base or conjugate acid‚ respectively) that permits solutions to resist large changes in pH upon the addition of small amounts of hydrogen ions (H+) or hydroxide ions (OH-). If the same amount of the buffer is added‚ the pH may only change a fraction of a unit. Our blood is a good example of a buffered system. It is maintained under a pH of 7.4. Thus‚ buffers are important in many areas of chemistry
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Eco-Column Lab Write up Alicia Whiteley Block 4 Abstract: In this research and experiment we were testing whether or not we can maintain a healthy eco-system based off creating one from scratch. We initially went into this experiment confident that we were going to be able to keep our fish alive which was proven wrong mid-way through due to high levels of turbidity and the state of the fish. After collecting all of our data‚ we maintained a healthy terrestrial level and a good PH
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Hair Conditioner Lab Intro The hair industry has been been devoted to answering a need of our american people for years. A market mainly targeted towards women‚ the search for products that will soften‚ repair‚ rejuvenate‚ enlarge‚ and even strengthen hair is unending. The question that we posed for this lab was: Does hair conditioner really actually strengthen hair as it promises? Materials Conditioner Plastic cup Forceps Hair (from Nate‚ Alyssa‚ Makenna‚ and Lisa) Microscope Water
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Erin Hillman August 30‚ 2010/5th period Biology Bunsen Burner Introduction: One of the most efficient ways to heat materials is by a Bunsen Burner. Bunsen Burners are made in a variety of different shapes and sizes. Hypothesis: In this lab my class learned the most efficient and safest way to use a Bunsen Burner. Materials: The materials used were; safety goggles‚ safety apron‚ ring stand‚ Bunsen Burner‚ flame striker‚ gas‚ wire gauze‚ a beaker‚ beaker tongs and 100 mL of water.
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Preparation of buffer solutions 1. Activation buffer (Mixed Phosphate Buffer‚ pH 5.5) Solution 1: An accurately weighed quantity of 1.61 g of potassium dihydrogen phosphate was dissolved in sufficient deionized water to produce 100 mL of solution. Solution 2: An accurately weighed quantity of 35.81 g of disodium hydrogen phosphate was dissolved in sufficient volume of deionized water to produce 100 mL. Accurately measured volume of 96.4 mL of solution 1 was mixed with 3.6 mL of solution 2 to get
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Procedure: Day 1: Buffer preparation First‚ the buffer was prepared by using the formula as follows: Figure 1: Calculation for prepare 0.5 M Tris buffer at pH 6.8 3.033 g of Tris was weighed and placed in 400 mL beaker. Then‚ 25 mL of distilled water was added into the beaker that contained Tris. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution
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