Chem 17 ▪ General Chemistry Laboratory II Experiment 1 Calorimetry INTRODUCTION Chemical reactions are usually accompanied by the evolution (exothermic reaction) or absorption (endothermic reaction) of heat energy. When measured at constant pressure‚ the heat evolved (qp < 0) or absorbed (qp > 0) is equal to the enthalpy change‚ symbolized by ΔH. ΔH is positive for an endothermic process and negative for an exothermic one. If H f is the enthalpy of the final state and Hi of the initial state
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Abstract: In the course of this experiment the rate of isomerism for the coordination complex cis[Co(en)2Cl2]Cl was determined using UV/Vis spectrometry. Using a range of wavelengths the optimum spectrometer setting for analysis was identified. The corresponding maximum and minimum absorbance of the cis and trans isomers respectively at 540 nm meant that it was selected as the wavelength to determine the rate of conversion between the isomers. The first order rate constant was calculated to be 0
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Firstly‚ the buret must be cleaned thoroughly with tap water. While cleaning the buret‚ it is also checked to make sure there are no leaks. The ring stand is then set up with a buret clamp and the cleaned buret placed in it. Then the buret is filled with 5-10mL of sodium hydroxide‚ M .0466 NaOH‚ three times and emptied after each time to completely rinse the buret. The buret is now filled will NaOH until it reads at the 0.00mL mark on the buret. The initial volume of NaOH in the buret is then
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Solution | Trial 1 | Trial 2 | Final buret reading‚ EDTA (mL) | 18.5 | 36.7 | Initial buret reading‚ EDTA(mL) | 0.5 | 18.5 | Determination of % MgO of Unknown Unknown Number | 4J | | Sample 1 | Sample 2 | Sample 3 | Mass of sample (g) | 0.2135 | 0.2132 | 0.2139 | Final buret reading‚ EDTA (mL) | 73.5 | 74.2 | 74.2 | Initial buret reading‚ EDTA(mL) | 0.5 | 0 | 0 | Measurement of water blank Final buret reading‚ EDTA(mL) | 14.5 | Initial buret reading‚ EDTA(mL) | 14.2 | Sample Calculation:
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principle of the lab. The experiments have all been tested thoroughly and tried by hundreds of students who have been successful. The experiments have been designed so that they can be completed within the allocated time with enough time to wash all glassware‚ submit data sheets and clear the bench tops. Your success in these lab sessions will depend on your ability to understand the experimental procedures and organize yourself so that you make efficient use of your time. IT IS THEREFORE IMPERATIVE
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2.5% acetic acid (15:85) on a reversed-phase column at ambient temperature. Elution was isocratic with UV detection at 257nm. Internal standard calibration method was used for quantitation with caffeine as the internal standard. Mean retention times for paracetamol and caffeine were respectively 2.61 ± 0.13 min and 11.98 ± 0.72 min . The calibration curve was linear over the range 0.1-5.0μg/ml. The method was also suitable for the assay of paracetamol-codeine combination drug as well as estimation
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Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
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indicator menu is available under the chemicals main menu (ChemicalsIndicators) or the context menu. Step 3: Fill buret with NaOH‚ obtain a 50 ml buret and fill with .100M NaOH solution. Step 4: Titrate NaOH into HCl until end point‚ record initial buret volume and add NaOH (quickly at first then slowly) until the HCl solution turns pink and record the final buret volume of NaOH in buret. Step 5 repeat steps 1-4 using pH meter‚ add a pH meter to the acid solution. Record numerous points of pH
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200ml beaker Measuring cylinder (10ml‚ 100ml) Pipette Burette Conical Flask Bunsen burner Any reagents used in this experiment Eggshell NaOH 1moldm3 HCl 1moldm3 Phenolphthalein Procedure 1. Each student should obtain one egg and the necessary glassware from the side bench. 2. Break the egg into a beaker. Add water to the egg and stir before pouring down the drain. 3. Wash the shell with deionized water and peel off all of the
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Fermentation of a Carbohydrate: Ethanol from Sucrose Abstract The purpose of this lab was to demonstrate the fermentation process of ethanol from the substrate sucrose. To make ethanol from sucrose two enzymes invertase and zymase were used. Vacuum filtration and fractional distillation were performed to get a more concentrated solution. The density of ethanol was .825 g/mL with a percent composition of 85% pure
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