Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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Analytical Chemistry I 1 Course Outlines LU1 :Analytical Chemistry Overview LU2: Measurements and Data Treatment LU3: Stoichiometric Calculations LU4: General Concepts of Chemical Equilibrium LU5: Gravimetric Methods LU6: Acid-Base Equilibria LU7: Acid-base titrations LU8: Analytical Separations Course Assessment Laboratory reports (15%) Assignments (15%) MidTerm Examination (30%) Final Examination (40%) 3 References 1. Christian‚ G.D. (2003)
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Crystallization and Melting Point Section: A61 Laboratory Exercise #1 Purification of a Solid: Crystallization and Melting Point Introduction In this lab exercise‚ we will be learning experimental techniques using glassware and other apparatuses. In order to successfully complete this lab‚ we will use techniques 1.0‚ 1.1‚1.2‚2.0‚2.1‚2.2‚ and 2.3 that are described in the Lab Manuel. In addition‚ we will learn how to determine the melting point of a solid through use
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experiment was to verify the molar volume of a gas and gas constant.¹ The method in which was used to determine the volume of H2 gas at standard temperature and pressure and the gas constant was to measure a strip of magnesium and place it inside the gas buret which contained hydrochloric acid‚ blue dye and distilled water and allow the reaction to occur. Once the reaction was complete and there were no visible gas bubbles‚ the volume was obtained and recorded. This procedure was completed three times.
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Titration analysis of unknown acids and bases (antacid tablets) Standardization of NaOH Materials: -Buret -250 mL Erlenmeyer flask -0.1 M NaOH -0.8 g KHP -Water -Three 150 mL beakers -magnetic stirrer A buret was rinsed with water and then with a small amount of the NaOH solution. Then the buret was filled with NaOH. The initial volume was read and recorded and the buret was labeled as BASE. Then approximately 0.8 g of KHP was placed in the flask. 100 mL of water was added and
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1.0 %‚ 1 mL Potassium hydrogen phthalate‚ KHC8H4O4‚ 2 g sodium hydroxide solution‚ NaOH‚ 0.1 M‚ 150 mL Unknown weak acid‚ 1.5g Water‚ distilled or deionized * Equipment: Balance Stir bar Beaker‚ 250mL Oven Buret‚ 50 mL pH sensor Desiccator Rising stand and buret clamp Erlenmeyer flask‚ 125mL Wash bottle with distilled water Funnel Weighing dishes‚ 2 Procedure: Part 1: Standardization of a Sodium Hydroxide Solution 1. Obtain a sample of potassium hydrogen phthalate that
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a clean beaker and then from the beaker into the volumetric flask or graduated cylinder. This will minimize contamination of the parent liquid source. Dispose properly of any leftover liquid. Do NOT pour it back into the original container. Why? This is so the original liquid does not get contaminated. When using a pipet or dropper to measure liquid‚ pour an aliquot into a clean beaker and then draw up the liquid from the beaker into the pipet. NEVER try to draw up chemicals by mouth. Why? Chemicals
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this lab was to determine the amount of grams of sodium bicarbonate (NaHCO3) required to produce enough CO2 gas to completely fill the lab and also how many Alka-Seltzer tablets that would equate to. This was done by collecting CO2 gas by inverting a buret and submerging it under water in order to calculate the volume of CO2 released from a fragment of Alka-Seltzer tablet. The main component of Alka-Seltzer is sodium bicarbonate‚ used to neutralize excess stomach acid during illness through the following
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Once this happens the titration is done. Record the final level of the iodine solution remaining in the buret‚ after titration. The difference between the initial level and the final level is the amount of iodine titration solution needed to create a reaction with the vitamin C. Rinse out and dry the Erlenmeyer flask. Repeat step 17-26 three times with the
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Introduction Titration is a process of neutralization and is commonly used to determine the concentration of an acid or base in a solution. This process involves a solution of known concentration delivered from a buret into the unknown solution until the substance being analyzed is just consumed. In this lab‚ experimenters using hydrochloric solution to react with the solution of NaOH according to this equation: HCl (aq)+ NaOH(aq) → NaCl(aq) + H2O(l) HCl is
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