Abstract: This experimentation was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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ab reportChemistry 117L Laboratory Report Name: Aneesa Noorani Lab Day: Tuesday Lab Room: SCL 114 Date of Experiment: January 22‚ 2013 TA: Mikhail 1. Basic Laboratory Skills Purpose(s) of the Experiment: The purpose of the first part of today’s experiment is to establish the stoichiometry of the reaction between titrate oxalate (C2O42-) and permanganate (MnO4-). The purpose of the second part of today’s experiment is to learn about the concepts of the rate of chemical reactions
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Enzyme Lab Daniyal Abdali (Rachel Lee) (Sarina Dolch) SBI 3UI Mr. Vrabec October 20‚ 2009 Test #1 * Add a small piece of cracker in test tube #1 and add Lugol’s solution. Observation #1 * The cracker turned a black colour when the Lugol’s solution was added to it. This was a positive result‚ meaning that the cracker contains starch. Test #2 * Add a bigger piece of cracker in test tube #2‚ add 5 mL of Benedict’s solution‚ place in a boiling water bath‚ and record observations
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expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate that responds with an enzyme is used to view the antibody/protein complex. Within a muscle tissue‚ there could be as many as 19 different
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Enzymes are organic catalysts‚ usually proteins that speed up metabolic reactions. They lower the amount of energy needed for reactions to progress in cells. In enzymatic activity‚ the molecules at the beginning are called substrates. Lactose metabolism is when lactose is destroyed‚ maintained or produced. For instance‚ being lactose intolerance that’s where lactose is destroyed. Metal cofactors in enzyme activity are required to function properly. The Effect of Temperature on Enzymatic Activity:
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6 November 2012 SUBJECT: Lab Report Analysis This memo proposes the observations and claims I collected from reviewing three different lab reports. Three fields of study are composed within this memo that includes Electrical Engineering‚ Environmental Engineering‚ and Petroleum Engineering. “Electrical Filters‚” (Electrical)‚ written by Joe Schmoe‚ is a lab report made by a student at a university. The College Board produced an environmental lab report named‚ “Monitoring Air Quality‚”
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The temperature of water was determined by a thermometer. 1 cm stripped ends of the wires was hooked into the input posts on the back of the impendence converter. Lab tutor module was started after. B. Baseline heart rate The top of the container was covered with foil and the animal was allowed to sit quietly for 10 min. The trace was examined by pressing start. After that the baseline or the controlled
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The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed
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