Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of 4oC‚ Room Temperature‚ 32oC‚ 48oC and boiling; then‚ at pH 3‚ pH 5‚ pH 7 and pH 9; and‚ finally‚ with the
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|4.8 |6 |3.3 |11 |4.2 |8 |10 |13 | | 1. The effects on water from hcl is that the ph went 1000x weaker than it went 100x weaker than it just didn’t change anymore. The effects on water from the NaOH are that it started with a 4 pH than went up to a 7 pH and it started to just keep the same pH. 2. The average pH change for the 30 drops of HCl added to the biological material is 4.3. 3. The average pH change for the 30 drops of NaOH added to the biological material is 8.3. 4. (Q2/Q3)
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oxides CO₂‚ SO₂ and NO₂ by using data that was collected using a pH sensor which was connected to a laptop with LoggerPro and Vernier. We created the gases by bubbling each gas into the water to create the three acidic solutions we were looking for. Once that step was completed‚ the pH levels of all three solutions were compared to each other to see the different strengths of each solution. As a result we found that NO₂ had the highest pH level change out of all the three gasses that we collected.
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amount 0.1 M NaOH used to lower/raise the pH of a blood buffer. In this experiment‚ HCl (a strong acid) and NaOH (a strong base) are used as examples of strong acids/bases‚ and the titration with H2PO4 shows the effect on a buffer solution. The assumption was the addition of large amounts of HCl will lower the pH‚ while the addition of large amounts of NaOH will increase the pH‚ while small amounts of either strong acid or base will not affect blood pH because of the buffer in blood. Human
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each region to the pH results obtained. - There are four significant regions in each titration curve‚ namely the initial‚ pre-equivalence‚ equivalence‚ and post-equivalence points. These points are named according to its position relative to the equivalence point. The equivalence point signifies the volume of titrant at which the solution becomes neutral. This is represented in the graph as the drastic change in pH‚ first with a sudden increase and a sudden decrease of pH. At the initial point
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Investigating the effect of pH on amylase activity This practical allows you to: * discover how pH affects the rate of an enzyme controlled reaction * evaluate the experimental procedure Procedure SAFETY: Follow your teacher’s instructions for handling the solutions. Wear eye protection when handling the iodine solution. Investigation * Place single drops of iodine solution in rows on the tile. * Label a test tube with the pH to be tested. * Use the syringe to place
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| | |the digestive system | | |The maximum pH measured during hyperventilation was _______. |7.67 | |The tidal volume (TV) when breathing at rest was about _____ ml. The TV with hyperventilation was
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which generally contain the volume of the titrant as the independent variable and the pH of the solution as the dependent variable (because it changes depending on the composition of the two solutions). The equivalence point on the graph is where all of the starting solution (usually an acid) has been neutralized by the titrant (usually a base). One can easily find the pKa of the monoprotic acid by finding the pH of the point halfway between the beginning of the curve and the equivalence point‚ and
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Effects of Temperature‚ PH‚ boiling and concentration on Horseradish Peroxidase ABSTRACT The purpose of this report is to find out the effect of change in the Temperature‚ PH‚ boiling‚ concentration in peroxidase activity. Peroxidase is an enzyme that converts toxic hydrogen peroxide (H2O2) into water and another harmless compound. In this experiment we use‚ turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change
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seconds‚ pH = 7.4 2. At 40 seconds‚ pH = 7.4 3. At 60 seconds‚ pH = 7.4 4. Did the pH level of the blood change at all during normal breathing? If so‚ how? The pH did not change during the normal breathing. 5. Was the pH level always within the “normal” range for the human body? Yes it was. 6. Did the PCO2 level change during the course of normal breathing? If so‚ how? No it did not change either. Activity 2a: Hyperventilation – Run 1 1. At 20 seconds‚ pH = 7.48 2. At 40 seconds‚ pH = 7
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