What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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TRAVEL GRAPH JULIANE AUTUMN DAMASCO IV-ST.JOHN BOSCO Dylan needs to visit his Grandparent’s house because it’s his Grandmother’s birthday. Since Dylan is used to commute‚ he traveled there riding public vehicles. He left the house at 8:00 am‚ he walked from his house to the Jeepney Station to ride a Jeepney going to Cubao. When he arrived at the Jeepney Station he‚ rode a Jeepney going to Cubao. He arrived at a Tricycle Station in Cubao and he left there riding a
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We are studying the effect of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure
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Basic Biochemistry CP2085 Effect of Temperature‚ Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature
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The Effects of varying p-Nitrophenyl Phosphate concentrations on Alkaline Phosphatase Activity Nathan Overholt Louis Settembrino March 5‚ 2002 Abstract: The enzyme Alkaline Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time assay was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm‚ and the Km was found to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction:
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LINEWEAVER-BURK PLOT A graph of the double reciprocal equation is also called a Lineweaver-Burk‚ 1/Vo vs 1/[S]. The y intercept is 1/VMAX; the x-intercept is 1/ KM; and the slope is KM/VMAX. Lineweaver Burk graphs are particularly useful for analyzing how enzyme kinematics change in the presence of inhibitors‚ competitive‚ noncompetitive‚ or a mixture of the two. There are three reversible inhibitors: competitive
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The bar chart illustrates the unemployment rate in Germany compared to the unemployment rate in Ireland. The y-axis shows the rate of unemployment which is defined by the number of unemployed persons as a percentage of the labor force. On the x-axis you can read the year. In 2000‚ 8 per cent of the German workforce was unemployed‚ which was twice as high as Ireland. Unemployment in Germany declined slightly in 2001. However‚ after this the unemployment rate rose steadily over the next four years
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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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Effects of Changes in Physical Properties on Enzyme Activity By Alex Hoffmann First Individual Lab Report Wednesday 7:30-10:15pm 10/24/12 Meghan Duell Abstract The goal of this lab was to determine the effects of certain physical properties on enzyme activity. Enzyme activity was measured by the height of the bubbles that appeared after the enzyme was added which are proportional to the rate of the reaction when time is constant. The fact that enzyme activity is affected by physical
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The role of salivary amylase in the digestion of starches remains controversial. In the absence of pancreatic amylase‚ the key enzyme for starch digestion‚ salivary amylase may well represent a potential compensatory alternate pathway for the digestion of amylose‚ amylopectin‚ and glycogen. Clinically significant depression of pancreatic amylase occurs in chronic pancreatitis‚ pancreatic resection‚ pancreatic neoplasm‚ cystic fibrosis‚ and other causes of pancreatic insufficiency. Quantitation
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