This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation technique thin layer chromatography and measuring the melting temperatures. The theory of column chromatography is
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Introduction In a chromatographic separation process‚ different kinds of functional groups and number of functional groups of molecules will determine the separation. Depending on what kind of solute‚ solvent‚ and adsorbent used‚ molecules will experience the interaction during dynamic equilibrium. On a TLC plate‚ capillary tube is used to transfer o- and p- hydroxyacetopheone‚ taking advantage of capillary force to make small spotting. A 30:70 ether acetate: petroleum ether is used as a solvent
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Introduction: The purpose of this lab was to separate and purify a compound. This was achieved using techniques that allowed the extraction of the acid‚ the isolation of the neutral compound and the melting point classification of the neutral compound to test the purity of the sample. Chemical Reactions: HA + OH- A- + H2O R-COOH + OH- R-COO- + H2O Organic Acid Insoluble in H2O Conjugate base Soluble in H2O Procedure: A 0.170g sample consisting of a mixture of 0.110g
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Theoretical Ratio | Obs. Ratio | Obs.m.p. | Physical Appearance | Ethyl 4-aminobenzoate | .233g | .2093 | 89% | 30% | 20% | 89 ° C | Off white appearance; powdery | Benzoic Acid | .233g | .243 | 104% | 30% | 20% | 125 ° C | Clear crystals | 9-fluorenone | .311g | .623 | 200% | 40% | 60% | 82 ° C | Yellow‚ filmy‚flakes | Discussion: Based on the results‚ the most accurate extraction achieved was the first compound‚ ethyl 4-aminobenzoate. With an 89% actual yield of the compound‚ a 3rd or 4th
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When the actual FD&C dyes in the green Kool-Aid were revealed‚ it was discovered that the initial assumption that Yellow 5 and Blue 1 are in the Kool-Aid was correct. However‚ solely based upon the Rf values‚ the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However‚ the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because
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Introduction Extraction involves dissolving a compound or compounds either from a solid into a solvent or from a solution into another solvent. Extraction is a method used to purify a substance by removing the impurities that was added to it. A solvent- solvent extraction is a type of extraction that is most commonly done by using two substances that are typically insoluble with each other. An acid-base extraction is a solvent-solvent extraction‚ in almost every case one of the solvents is water
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Date: Lab Section: Selected Known Compounds Name Structural Formula Literature Melting Point Acetanilide C8H9NO 114.3°C Fluorene C13H10 116.5°C Experimental Data Sample Composition 1. 100% Acetanilide 2. 10% Acetanilide 3. 25% Acetanilide 4. 50% Acetanilide Fluorene 5. 75% Acetanilide 6. 90% Acetanilide 7. 100% Fluorene Melting Range 108-116°C 108-112°C 105-112°C 102-109°C 105-112°C 109-115°C 108-114.5°C Graph of Data Discussion of Results The purpose
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of this experiment was to oxidize an alcohol (9-hydroxyfluorene) to a ketone (9-fluorenone) using aqueous sodium hypochlorite (bleach) as the oxidizing agent‚ while introducing techniques used in microscale experiments. Reaction: Results 1. Recrystallized Product Yield Product yield = (actual yield/theoretical yield) x 100% 3mL 9-hydroxyfluorene x (1mL/1000mL) x (0.09 mol/L) = 2.7 x 10-4 moles 0.05g 9-fluorenone / (180.20g/mol) = 2.77 x 10-4 moles Product yield: (0.000277 mol/0.00027 mol) x
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yielding a 95.8% recovery rate. As for the organic compound‚ 0.166g of fluorene was secured and its respective melting point range was 116.0 C – 117.0 C. The identity of final acidic product was revealed through the comparison in melting points of the three known products. The melting point range matched up similarly with 3-chlorobenzoic acid. The experiment began with 0.210g of the mixture of an unknown acid and fluorene. The yield in products
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made me think that we did something wrong and our results would not be accurate. Crystallization: Part B: Start Finish % Recovery Expected Remaining Sulfanilamide 0.093g 0.059g 68.6% 0.034g Part C: Start Finish % Recovery Expected Remaining Fluorene 0.044g 0.018g 48.6% 0.026g Melting Points: Compound Literature Melting
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