Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography
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Taste discrimination in young and old Drosophila melanogaster Introduction The ability to discriminate taste is critical for the survival and nutrition of an organism (Sellier et al.‚ 2010‚ Gordesky-Gold et al.‚ 2008). Many animals rely on their taste organs to detect and discriminate various types of taste such as sweet or bitter (Weiss et al.‚ 2011). When these organs come in contact with a food source‚ signals are sent to taste receptor cells. The activation of certain taste receptor cells by
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Chromatography • • • Separation based on polarity of compounds Two potential phases for a compound to exist in: mobile and stationary Partitioning of compounds between mobile phase and stationary phase occurs: o Compounds that are less polar move more in the mobile phase‚ those that are more polar “stick” more on the stationary phase o These polarity differences cause compounds move at different rates and therefore can be separated 1. Mobile Phase: the phase the moves; can be gas or
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Anionic Exchange Ion Chromatography to Detect the Anions Concentrations in the water Samples Narendra Boppana‚ Nasser Ali‚ Martin Chavez‚ Sear Introduction: Ion Exchange Chromatography is a process where the ionic exchange occurs in between the stationary phase and the sample in the mobile phase‚ by using this ionic exchange phenomenon it is easy to separate the compounds. Ionic exchange depends on the electrical properties
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An Eye For An Eye Capital punishment has often been referred to as ‘an eye for an eye’. This idiomatic expression dates back to Mesopotamian times from Hammurabi’s Code. The phrase represents what many view as a harsh sense of justice based on a need for reprisal. What is the real purpose for capital punishment? Many Americans are becoming less and less concerned with using punishment as a deterrent for future crimes and more focused on using it as a means of retribution. In a recent study‚ eighty-eight
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An Eye for an Eye Life is a precious gift from God. Even when a person has no material possessions‚ life is still possessed. In light of these observations‚ it is logical to assume that murder‚ the taking of another’s life‚ is the most heinous of crimes. Undeniably‚ penalties imposed upon criminals should match the crimes committed. Therefore‚ the worst crime possible‚ murder‚ should receive the worst penalty possible‚ death. One argument against the death penalty comes from the Bible‚
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The purpose of this lab was to use gas chromatography to determine the relative amounts of the three components of gas products from the dehydration reaction of 1- and 2-Butanol with sulfuric acid and the dehydrobromination reaction of 1- and 2-Bromobutane with potassium tert-butoxide. E1 reactions are two-step unimolecular reactions. The first step of an E1 reaction occurs when a leaving group‚ which consists of a weak base‚ leaves an organic compound‚ leaving an intermediate carbocation. In the
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Chromatography is the separation of compounds to allow us to see the different colours of that compound‚ for instance we may use chromatography in a school lab to see the real colour compounds of plants and grass‚ by using chemicals such as acetone we it will allow the object to break compounds and thus we can see the true colour of the grass. In today’s society there are many real world applications which use the chromatography technique in their line of work‚ an example of one of these applications
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Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
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