The total protein content was estimated as proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2‚ 0.4‚ 0.6‚ 0.8‚ 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes‚ 0.1 and 0.2 ml of the extract were taken. In each test tube‚ the volume was made up to 1 ml‚ followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left
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hydrous copper sulfate needs to be determined. Clean the glassware then get four 50 mL Erlenmeyer flasks with 25 mL of the stock solution and aqueous solvent. Prepare all the unknown sample solutions for determining Beer’s Law Graph. Turn on colorimeter at 100% T then calibrate. Get the data and analyze it if it is correct. Experiment 3 – Percentage of sulfate in sample. Weight out the sample and dissolve it. Heat it and then slowly add the precipitating agent. Heat it for about 30 minutes while
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determine the accuracy of the copper and zinc composition percentages in a random sampling of pennies. The penny was dissolved to make aqueous copper ions and four copper dilutions were made from stock solution. Each cuvette sample was measured in a colorimeter and the data was plotted linearly using Beer’s law plot. Mass percent and percent error were found using calculations. Analysis of class data provided further data to determine the copper and zinc composition percentages. Experimental Procedure
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activity). Algal bloom is the direct result of eutrophication where suffocation of aquatic life and nutrient depletion may occur. Professionals may use a colorimetric analysis to measure the absorption of light from coloured water sample using a colorimeter to determine the concentration of nitrate and phosphate present. An acid base titration can also convert nitrogen into ammonia to examine the result. It is
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this reaction. Hypothesis: If the reaction between crystal violet and sodium hydroxide reacts appropriately‚ then the order will be first order. Procedure: Mix 10 mL of sodium hydroxide and crystal violet solution together. After calibrating the colorimeter‚ insert the mixture. Absorbance data will be collected for three minutes. Analyze the data graphically to determine the reaction. Discussion: The hypothesis that I stated was sadly‚ not supported by the data that my partner and I obtained. Comparing
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Support Material Exemplar Starter Pages GCE Chemistry B (Salters) OCR Advanced GCE in Chemistry B (Salters): H435 Unit: F336 Chemistry Individual Investigation This Support Material booklet is designed to accompany the OCR Advanced GCE specification in Chemistry B (Salters) for teaching from September 2008. Contents Contents 2 Support Materials 3 Choice of investigation topic 4 Health and Safety 33 Other forms of support 35 Support Materials These support materials should
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which the absorbance is the at the greatest. [pic] INTRODUCTION A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the solution. A spectrophotometer differs from a colorimeter in the manner in which light is separated into its component wavelengths. A spectrophotometer uses a prism to separate light and a colorimeter uses filters. Both are based on a simple design of passing light of a known wavelength through
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Purity of Aspirin Objectives - To research‚ using various sources‚ the history of aspirin‚ its use in medicine‚ methods of synthesizing it and of measuring its purity - To compare the % purity of a branded aspirin tablet with a generic aspirin tablet - To compare 2 methods of composition analysis of the two types of aspirin Research
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Day one consisted of cutting the beetroots and placing them at each temperature while day two was used to measure how much green light was absorbed by the colorimeter. This could have affected the results because light could have reached the tubes causing some of the pigment to be damaged and not give accurate results. There are not many solutions for this but I suggest that we perform the lab experiment with
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which differs greatly from the specification. Possible sources of this error are * Using a measuring cylinder instead of a volumetric flask when diluting * Contaminated test tubes * 2. If this analysis was performed using a colorimeter‚ yellow light would be used to measure the absorbance of solutions. Why is blue light not used? Blue light cannot be used to measure absorbance of the solution because it cannot be absorbed. Instead of absorbing‚ the solution reflects blue light
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