LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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This reaction is carried out by adding benzaldehyde and acetone into a flask. The product created is a 1‚5-diphenyl-1‚4-pentdiene-3-one‚ which includes two double bonds‚ and two benzyl ring functional groups. This is a dehydration reaction that occurs twice in order to form the diene. After obtaining the product‚ via vacuum filtration‚ it will be recrystallized and then analyzed for purity by determining both products’ melting point. The two products will be compared. Chemical Reactions:
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Solute Concentration of Potatoes Lab #1 Purpose: To find the molarity/concentration of potato cytoplasm. Materials: As on page 1 in the lab handout. Procedure: As on page 1 in the lab handout. Data and Observations: Test Tube # | Concentration of sucrose solution (mol/L) | Initial Mass (g) | Final Mass | Percentage change in mass | 1 | 1.0 mol/L | 3.00g | 2.25g | -25.0% | 2 | 0.9 mol/L | 2.70g | 2.07g | -23.3% | 3 | 0.8 mol/L | 2.92g | 2.25g | -22.9% | 4 | 0.7 mol/L | 2.60g
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The Effects of Individuals’ Detection of Changes in Images on Reaction Time Abstract The purpose of this current study was to examine how individuals detected or noticed change when viewing images on their level of attentiveness. The experiment consisted of 22 participants who had to detect change across conditions for 20 minutes. These conditions were importance of change (marginal and central) and change type (color‚ location and disappearance and reappearance of images). These
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our readings were in milliliters so we had to convert first. In conclusion‚ even though the lab procedure is not about moles‚ most of the calculations are and this is one lab where the concept of
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
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Corporate Communication Assignment CCOM5112 Dimpho Mabulana Student Number: 13006850 Lecturer: A3 Human “Public relations (PR) is the practice of managing the spread of information between an individual or an organization and the public. Public relations may include an organization or individual gaining exposure to their audiences using topics of public interest and news items that do not require direct payment. The aim of public relations by a company often is to persuade the public‚ investors
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atom or a fluorescent dye is used as label on probe. Reverse Transcription Polymerase Chain Reaction (RT)-PCR Employing PCR-based procedure in analyzing RNA would require cDNA to be synthesized using reverse transcription. When reverse transcription and polymerase chain reaction are combined together‚ it will permit detection of low abundance RNAs. RT-PCR is distinct from Real Time Polymerase Chain Reaction (qPCR). The latter measures the amplification of DNA in quantitative way and it uses fluorescent
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Introduction: The purpose of this lab is to apply Mendel’s laws to predict the probability of the occurrence of a single event‚ of two independent events and of certain traits in offspring of parents exhibiting traits. Gregor Mendel was an Austrian monk in 1866‚ who studied how traits were passed using pea plants. From his studies of inheritance‚ he created three laws of inheritance: the law of dominance‚ the law of segregation‚ and the law of independent assortment. He called genes ‘’factors’’
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