Bacterial growth patterns and the effects of environmental factors on properties of colonies ERIC LI Abstract In this experiment we work with different types of bacteria and experiment with their different properties and how they grow in certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Unit 3. Assignment 1: Bacterial Growth Rates 1. Mediums that could be used to determine shigellosis include Btilliant Green Agar‚ and Triple Sugar-Iron Agar. Expected results in a confirmed case of shigellosis are as follows: Brilliant Green Agar – Isolated Shigella colonies which do not ferment lactose or sucrose and appear red or white in color with no growth to trace growth on the Agar plate will be present. Triple Sugar-Iron Agar – Presence of Shigella will manifest as a red slant with
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Bacterial Growth Requirements Evelyn Lyle ITT Technical Institute Angela Ask‚ MPS January 15‚ 2012 Every organism must find in its environment all of the substances required for energy generation and cellular biosynthesis. The chemicals and elements of this environment that are utilized for bacterial growth are referred to as nutrients. Many bacteria can be identified in the environment by inspection or using genetic techniques. The nutritional requirements of a bacterium such as E Coli are
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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Fighting Bacterial Growth The purpose of this lab was to determine the effectiveness of antiseptics‚ disinfectants‚ and antibiotics on bacteria. The hypothesis was that if bleach was used‚ it would be the most effective because bleach is commonly used to clean and disinfect various things. The variables that were tested were antibacterial soap and Scope mouthwash for the antiseptics; bleach and ammonia for the disinfectant; and Cipro‚ erythromycin‚ and tetracycline for the antibiotics. All of these
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Vibrio natriegens’s generation time and optimal growth environment using a Closed-Growth System. Abstract: The objective of this experiment is to find out what the optimal growth environment is for V. natriegens out of three different environments. The first is a Brain Heart Infusion broth (BHI) that contains 50 mM of NaCl. The second is BHI containing 250 mM of NaCl. The third is BHI containing 1000 mM NaCl. In order to obtain this‚ a growth curve graph must be constructed for each different
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bacterium Escherichia coli is being subjected to various environmental factors that affect the rate of growth. These factors scrutinized were the different types of nutrients‚ the intensity of aeration‚ or the temperature at which it was stored. The purpose of this lab is to determine which factor affects the Escherichia coli the greatest. It is known that these abiotic factors affect the rate of growth the greatest if they remain at the correct conditions for living. Escherichia coli and other bacteria
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a comparative study on growth rate and lipid content of various microalgae samples collected from biologically and geographically diversified areas on Nepal. Comparisons were also made on samples with same genus isolated from geographically diversified areas (i.e. high altitude (>4000 m) and mid hill (>1000 m))‚ and effect of seasonal variation (with respect to temperature) on similar species of microalgae. Euglena sp.‚ Chlorella vulgaris‚ and Scenedesmus sp. with growth rate of 0.401 day-1‚ 0
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Bacteria Growth Requirements Microbiology Life as we now it has ended. What is left you ask? Well it is said the only thing that could survive an incident that could end our known way of life is a roach and a pack or Twinkies. In truth the great survivor would be microorganisms. Microorganisms can survive where most cannot due to their size‚ nutritional needs‚ energy requirements‚ and are very good at adapting to different environments (Black 2008). Microorganisms require two things to
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