Preview

Bacterial Growth Patterns

Powerful Essays
Open Document
Open Document
2046 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Bacterial Growth Patterns
Bacterial growth patterns and the effects of environmental factors on properties of colonies
ERIC LI Abstract In this experiment we work with different types of bacteria and experiment with their different properties and how they grow in certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth, shaded mulch, and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The bacteria were then left to grow on their own in a relatively well shaded area. As a control, a cotton swab that had nothing on it was used. It was recognized that different types of bacteria exist in different environments and grow in different ways compared with one another. As was discovered, the human mouth generally holds brightly colored bacteria varying from red to yellow that grow in a disc shape with no discernible repetitions or rings while other areas such as table tops and dirt had more darkly colored cultures forming in alternating rings of light and dark brown or gray. In addition, given the time available to grow, the average culture grew to a size of 1 cm in diameter ranging from 0.7 to 1.4 cm. Most cultures were found to be circular in shape thus implying constant growth in all directions. Knowing the different types of growth patterns will allow future doctors and medicinal practitioners to better know how to recognize certain bacterial growths and infections.

Introduction In the field of medicine today discerning different bacterial infections from one another is an important technique for doctors and medical practitioners. The difference in growth patterns of bacteria allows scientists to distinguish different bacteria from one another, especially when symptoms of infections are unclear. The experiment also gives information on how to control bacterial growth in the case of developing antibiotics and other medicines. The method of collecting bacteria



Cited: Bibby, Basil G., and George Parker Berry. "A Cultural Study of Filamentous Bacteria Obtained from the Human Mouth." Journal of Bacteriology 38.3 (1939): 263-74. US National Library of Health. Web. 2 June 2013. . Kuru, Erkin, et al. "In?Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with Fluorescent D-Amino Acids." Angewandte Chemie International Edition 51.50 (2012): 12519-23. Abstract. Wiley Online Library. Web. 2 June 2013. . Lacasta, A. M., et al. "Modeling of spatiotemporal patterns in bacterial colonies." Physical Review E 59.6 (1999): 7036-41. Abstract. American Physical Society. Web. 2 June 2013. . Matsushita, M., et al. "Interface growth and pattern formation in bacterial colonies." Physica A: Statistical Mechanics and its Applications 249.1-4 (1998): 517-24. Abstract. Science Direct. Web. 2 June 2013. . Mild, Kjell Hansson, et al. "Background ELF magnetic fields in incubators: A factor of importance in cell culture work." Cell Biology International 33.7 (2009): 755-57. Abstract. Science Direct. Web. 2 June 2013. . Tranvik, Lars J., and Manfred G. Hofle. "Bacterial Growth in Mixed Cultures on Dissolved Organic Carbon from Humic and Clear Waters." Applied and Environmental Microbiology 53.3 (1987): 482-88. American Society of Microbiology. Web. 2 June 2013. .

You May Also Find These Documents Helpful

  • Good Essays

    Specific bacterial morphologies noted in exercise one come from the spiral bacteria sample. The shape appears to be corkscrew in nature and vary in length throughout the sample slide. The length varies but the width seems to be uniform. The color most likely arises from the stain used to allow for better visualization of the organism. The Bacillus sample has no discernable morphology and appears as round ended, and cylindrical shaped in nature. The organisms have a black tint which is probably related to the type of statin used for visualization of the organism.…

    • 588 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Double Unknown Lab Report

    • 1229 Words
    • 5 Pages

    The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus aureus with a positive confirmatory test, mannitol salt agar, showing consistent results as well for S. aureus. The Gram-negative bacteria were Pseudomonas aeruginosa with a positive confirmatory…

    • 1229 Words
    • 5 Pages
    Powerful Essays
  • Good Essays

    Cited: Tortora, G., J. Funke, B.R., Case, C.C. (2010) Microbiology: An Introduction. Tenth Edition. San Francisco, Pearson Benjamin Cummings.…

    • 1198 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    Lab 4

    • 890 Words
    • 4 Pages

    Results/Analysis: Gained knowledge about culture media and how to distinguish various types of microbial growth. I also learn about variable conditions that are required for microbial growth, including oxygen levels and temperature.…

    • 890 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    Describe the precise chemical (or physical) reactions that produce these patterns of bacterial growth in each case. (That is, say WHY these results occur). Some of the information is available in your lab manual but for others you may have to do some research to find out the answers.…

    • 648 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    BIO20002 Prac Report 2 1

    • 915 Words
    • 8 Pages

    This experiment is aimed to examine the effects of environment such as Oxygen, Temperature, pH and Osmotic Limitations on the growth of a various kind of bacteria.…

    • 915 Words
    • 8 Pages
    Powerful Essays
  • Satisfactory Essays

    Bacteria grown in a closed system show a specific growth pattern called the growth curve which consists of four phases. The lag phase, which is a period of slow growth; exponential phase, period of maximum growth; stationary phase, where nutrients become the limiting factor making the growth rate equal to the death rate and the death phase where organisms die faster than they are replaced. It is important to know how fast a microbe grows in order to know bactericidal or antibiotic concentrations, temperature and ph at which they stop growing. Evaluating a growth curve gives you a perspective of the generation time and the mean growth rate constant which would help you estimate the minimum, maximum and optimum growth temperature of the microbe. In this experiment you would be able to see the duration of each phase, the mean growth rate constant (k) is used to measure how fast cells are dividing in a culture, generation time, optical density (OD) which s the measure of the amount of light absorbed by a suspension of bacterial cells, and the organism’s minimum, maximum, and optimum temperatures…

    • 1189 Words
    • 5 Pages
    Satisfactory Essays
  • Good Essays

    Bacteria can be distinguished from one another by their morphology (size, shape, and staining characteristics). In this lab experiment, bacterial morphology was examined by observing both stained and unstained organisms.…

    • 533 Words
    • 3 Pages
    Good Essays
  • Better Essays

    In fact, in these two samples, bacterial growth covered the entire Petri dishes. However, a large number of bacterial colonies manifesting the most significant variances in their morphologies…

    • 990 Words
    • 4 Pages
    Better Essays
  • Better Essays

    Microbio lab report body

    • 2621 Words
    • 11 Pages

    This report contains the background information on gram positive and gram negative bacteria, which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of identification used are also described in this report to give an explanation as to why a certain route was taken in carrying out experiment 14. The results of the experiment carried out for the identification of three unknowns are tabulated and then these are discussed to give an explanation as to why such results were gotten and what the results show to prove the identity of a certain bacteria. The bacteria identified in unknown #16 are: Klebsiella pneumonia, Staphylococcus epidermidis and Yersinia enterocolitica.…

    • 2621 Words
    • 11 Pages
    Better Essays
  • Good Essays

    Bacteria

    • 644 Words
    • 3 Pages

    By using aseptic, a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony, which is pure colony.…

    • 644 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Unknown Microbiology

    • 472 Words
    • 2 Pages

    There are several different medical reasons for identifying microorganisms. The reasons may vary from having to know the causative agent of a disease in a patient to be able to treat and care for them properly, to knowing the correct microorganism to be used for making certain antibiotics as well as proper dosages, down to knowing all microbes associated with consumed foods such as plants and animals in case of an allergen or a contamination outbreak. This analysis was done by utilizing all of the proper methods taught thus far in the microbiology laboratory for the identification of two unknown bacteria.…

    • 472 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Observing Bacteria

    • 1135 Words
    • 5 Pages

    Purpose: The purpose of this lab was to display knowledge of the use of a compound microscope with and without an oil immersion lens while observing and identifying various bacterial shapes and arrangements, including a self prepared yogurt culture.…

    • 1135 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Unknown Bacteria

    • 1076 Words
    • 5 Pages

    Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons, such as knowing the causative agent of disease, knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study. This study allows the student to exercise their ability to use and understand sterile techniques, transmission, and prevention of microorganisms.…

    • 1076 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Bacteria Ecology

    • 744 Words
    • 3 Pages

    This lab experiment serves as a model for community succession using bacterial colonies as the model. A bacterial colony grows from a single bacterium and is composed of millions of cells. Each colony has distinctive colony morphology: size, shape, color, consistency, and color. Community succession is a phenomenon observed in the organizational hierarchy of all living organisms. Community succession is not limited to bacterial colonies, but spans the entire community of life. As the community grows, it changes the environment it inhabits, and the resulting community is different than at the start. As community succession occurs in bacterial colonies pH, odor, color, and consistency changes take place. In this experiment, four different milk age samples, fresh, 24 hour old, 4 day old, and 8 day old milk, will be prepared on different sets of agar plates that will be diluted to different levels for optimum bacterial growth and measurement.…

    • 744 Words
    • 3 Pages
    Good Essays