1. Explain Why All The Bacteria Grow So Poorly In AMB
MCB 2010C Lab Report: Growth Media
1. State the difference between chemically defined and complex media in your own words. (2) Chemically define is a media that is pure and contains specific compounds from either organic or inorganic. Also, chemically define media is a chemical composition that is known. On the other hand, Complex media is a complex media that is unknown and is usually not a chemically pure.
2. Classify the media used (YEB, NB, GSB and ISB) as either complex or chemically defined. (4)
YEB
Complex
NB
Complex
GSB
Chemically define
ISB
Chemically define
3. Explain why all the bacteria grow so poorly in ISB? (1)
ISB is an inorganic synthetic broth in which bacteria is a heterotrophic organism that require …show more content…
Describe the precise chemical (or physical) reactions that produce these patterns of bacterial growth in each case. (That is, say WHY these results occur). Some of the information is available in your lab manual but for others you may have to do some research to find out the answers.
Medium
Description of chemical or physical reaction
E. aerogenes colonies on EMB are pink
E. aerogenes ferments lactose in the medium to produce acid. This lowers the pH and the dyes in the medium it turn pink
PEA prevents gram negatives from growing
(2)
PEA prevents the growth of Gram-negative organisms by disrupting the structure of lipids in the Gram-negative membrane. It also can hamper protein synthesis.
Hemolysis is displayed on blood agar plates
(2)
On the plate the bacteria produces enzymes called hemolysins these enzymes lyse the red blood cells to certain degrees. Bacteria the fully lyse the blood go through beta-hemolysis. Bacteria that partially lyse the blood go through alpha-hemolysis, and bacteria that doesn’t lyse at all goes through gamma hemolysis.
A yellow color develops around S. aureus on MSA
(3)
The pathogenic species of staphylococcus ferment mannitol and produce acid which turns the pH indicator
1. State the difference between chemically defined and complex media in your own words. (2) Chemically define is a media that is pure and contains specific compounds from either organic or inorganic. Also, chemically define media is a chemical composition that is known. On the other hand, Complex media is a complex media that is unknown and is usually not a chemically pure.
2. Classify the media used (YEB, NB, GSB and ISB) as either complex or chemically defined. (4)
YEB
Complex
NB
Complex
GSB
Chemically define
ISB
Chemically define
3. Explain why all the bacteria grow so poorly in ISB? (1)
ISB is an inorganic synthetic broth in which bacteria is a heterotrophic organism that require …show more content…
Describe the precise chemical (or physical) reactions that produce these patterns of bacterial growth in each case. (That is, say WHY these results occur). Some of the information is available in your lab manual but for others you may have to do some research to find out the answers.
Medium
Description of chemical or physical reaction
E. aerogenes colonies on EMB are pink
E. aerogenes ferments lactose in the medium to produce acid. This lowers the pH and the dyes in the medium it turn pink
PEA prevents gram negatives from growing
(2)
PEA prevents the growth of Gram-negative organisms by disrupting the structure of lipids in the Gram-negative membrane. It also can hamper protein synthesis.
Hemolysis is displayed on blood agar plates
(2)
On the plate the bacteria produces enzymes called hemolysins these enzymes lyse the red blood cells to certain degrees. Bacteria the fully lyse the blood go through beta-hemolysis. Bacteria that partially lyse the blood go through alpha-hemolysis, and bacteria that doesn’t lyse at all goes through gamma hemolysis.
A yellow color develops around S. aureus on MSA
(3)
The pathogenic species of staphylococcus ferment mannitol and produce acid which turns the pH indicator