Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates Inhibition of enzyme activity pH
Premium Enzyme Enzyme inhibitor Reaction rate
and analyzed various DNA fragments in order to determine if these DNA fragments originated from the same individual. The learning objective for this lab is to gain a better understanding of how DNA fingerprinting works. In this lab the primary function is to determine which DNA fragments match the DNA fragment found on the crime scene. To determine if any of the DNA fragments match the fragment found at the crime scene‚ the DNA fragments must undergo the DNA fingerprinting. DNA fingerprinting causes
Premium
FORENSIC DNA ANALYSIS Forensic serve both the living and the dead -- those who are victims‚ suspects‚ survivors‚ and those who are left behind. Forensic nursing blends nursing science with medicine‚ law‚ and criminology. At its core‚ this specialty seeks to address healthcare issues that have a medico legal component. Long before holding the designation of forensic nurse‚ nurses provided care to the victims and perpetrators of violence. History reveals that in the 14th century‚ performed gynecologic
Premium Forensic science Crime Sherlock Holmes
YOUR NOTES UNIT 2 NOTES DNA (deoxyribonucleic acid) DNA Functions • Stores genetic information and copies itself (replication) to pass on the information • Contains genes (instructions to make proteins) • Instructs cell’s activities DNA Structure • DNA is a polymer of nucleotides • Chromosomes (DNA strand + associated proteins ie. Histones wrap DNA around like a spool = condensed chromatin) ↓ genes (sections of a chromosome that codes for a protein) ↓ nucleotides (3 parts:
Free DNA
Objective: To investigate how pH affects the rate of protein digestion Hypothesis: I predict that since pepsin breaks down the material the quickest at a pH of 2 in the stomach‚ test tubes #3 will reduce the greatest because it has pepsin to further break down the egg. Whereas the other test tubes will have a smaller reduction. I think test tube #2 will have the second largest reduction rate and then #6 because they all will have a greater rate at breaking down the material‚ Then‚ I predict the
Free PH
3.0 Introduction The methodology involves five main steps; the collection of hair samples‚ the distribution of questionnaires‚ the washing of samples‚ the digestion of the samples‚ and the analysis of metal contents in the hair. 3.1 Materials and reagents used during the experiment The apparatus and chemicals that were used in the previously mentioned experimental steps are summarised in table 3.0. Steps Materials Reagents Collection of hair samples 1. Stainless Steel Scissors 2. Plastic bags
Premium Erlenmeyer flask Laboratory glassware Solvent
where all of the parts are located‚ like using mnemonics. Feel free to be creative.: Mouth Food begins its journey through the digestive system in the mouth‚ also known as the oral cavity. Inside the mouth are many accessory organs that aid in the digestion of food—the tongue‚ teeth‚ and salivary glands. Teeth chop food into small pieces‚ which are moistened by saliva before the tongue and other muscles push the food into the pharynx. • Teeth. The teeth are 32 small‚ hard organs found along the anterior
Premium Digestion Stomach Digestive system
Enzymes An enzyme is a protein used to speed up the rate of a chemical reaction. Because they regulate the rate of chemical reactions‚ they are also called catalysts. There are many‚ many different types of enzymes‚ because for each chemical reaction that occurs‚ an enzyme specific to that reaction must be made. To act on a substrate‚ an enzyme must contain an active site. The active site is the area on the enzyme that allows the substrate and enzyme to fit together. The amino acids that are present
Premium Enzyme PH
These restrictions are a concern within the physical therapy profession. Common limitations are number of visits‚ time per visit‚ restrictions on patient populations that have direct access‚ and requirements of previous diagnoses. Treatments available without physician referral are currently limited and physical referral is still mandated for certain treatments (Direct Access at the State Level‚ 2015). However‚ Montana law states there are no such restrictions and a licensed physical
Premium Physical therapy Patient Therapy
Gene Cloning Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from
Premium DNA