The Effect of Substrate Concentration on Initial Reaction Rate The purpose of this study is to identify the relationship between substrate concentration and initial reaction rate provided that substrate concentration is much greater than enzyme concentration. Enzymes are essential to life as they are required for many vital metabolic reactions to occur. To adequately explain the properties of enzymes‚ it is assumed that an enzyme-controlled reaction takes place through an enzyme-substrate
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sugars were found and not starch‚ is because saliva contains an enzyme known as salivary amylase which catalyses the breakdown of starch to produce sugars such as maltose (Sherwood‚ 2013). When testing the effect of the salivary amylase concentration‚ we observed that the solution containing the most saliva (3ml)‚ become colourless faster than the solution which contained the least saliva (1ml). This proves that an increase in enzyme concentration (increase in saliva)‚ will cause an increase in the
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The Behavior of Proteins: Enzymes Enzymes are Effective Biological Catalyst Catalysis- speeds up metabolism to allow production of products. Enzymes- Highly specific and most efficient catalyst that speeds up metabolism or rate of reaction in organisms by factor up to 10^20 (globular proteins) Nonenzymatic catalyst- enhance by 10^2 -10^4 Ribozymes- acts for catalytic activity in RNA’s Kinetics versus Thermodynamics Standard free energy change- difference between the energies of the reactants
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LABORATORY 3 The Effect of Temperature on the Rate of PNPP Hydrolysis Partners: Shelby Cruickshanks Alexis Williamson Introduction Most of the chemical reactions‚ which occur throughout our bodies‚ would proceed at a much slower rate of reaction without the presence of an enzyme. Cells can not wait for centuries for molecules to break down‚ if they waited for that‚ there would be no way for the organism to obtain energy or in turn survive. This is where biological catalyst comes into
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------------------------------------------------- Enzyme Pre-Lab Harry Kang 9/26/12 1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions‚ such as‚ different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with‚ 1.5 ml of H2O2‚ 1.5ml of H2O and different amounts of enzyme drops‚ to determine how much oxygen gas is produced during the reaction
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Enzymes are biological catalysts that operate at the temperatures‚ pressures‚ and moderate pH values found in living organisms. They help in a faster chemical reaction in our human body. Besides this‚ enzymes also play a huge role in various industries. The following paragraphs will talk about how various enzymes are used in various industries : The Dairy Industry and Medical Industry. In the dairy industry‚ the enzyme Protease is used widely. While making cheese‚ protease helps coagulate milk
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prepared for the experiment‚ one at room temperature and the other two at 15°C and at 5°C. The room temperature water bath was prepared by filling a basin with tap water about ¾ full so that when the subject’s face is submerged‚ the water will not spill. A thermometer was used in order to obtain the temperature of the water. The temperature obtained was at 26°C. The water baths having temperatures of 15°C and 5°C were also prepared the same way as the room temperature water bath only with the addition of
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FUNCTIONS OF ENZYMES Enzymes are extremely important to the human body and its ability to function. An enzyme itself is a protein made by the body’s cells to act as catalyst‚ speeding up chemical reactions in the cell. It does this by taking the reactants‚ or the elements or compounds that enter into the chemical reaction‚ in this situation referred to as substrates‚ and breaking apart their bonds so that new ones can form. The three types of enzymes are digestive enzymes‚ food enzymes‚ and metabolic
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Introduction: Enzymes are biological catalysts that permit cells to carry out the many functions that are required in a living system. Every enzyme has a specific substrate and a specific function. Enzymes alter substrates one of three ways: by adding something to the substrate‚ removing something from the substrate‚ or by changing its conformation‚ otherwise known as its shape. The structure of an enzyme and its ability to function exists because it binds to an active site‚ thus increasing the
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data. As evident in the table‚ the 10oC temperature is very accurate as the range was 0‚ resulting in an error percentage of 0%. However‚ the 35oC temperature had a no clear skew of results as indicated through the range‚ 14 and the error percentage‚ 161.47%‚ suggesting that the trials at this temperature were not very accurate as they were largely inconsistent. The 50oC also proved to have inaccuracies‚ however‚ not as significant as the previous temperature‚
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