enzymatic activity Background Information: Trypsin is a protease which conducts hydrolysis forming peptides. It is an enzyme which is secreted by the pancreas into the small intestine and works best in an alkaline environment. Egg white is used in this practical as it is found to contain the protein/ enzyme trypsin. pH is the measure of the amount of H+ ions in a solution‚ these ions affect the shape of the enzyme. Hypothesis: That as pH increases‚ the rate of enzymatic activity increases
Free PH
General Conclusion: These experiments helped us understand the importance of molecular polarity. This knowledge can now be used for the future to predict‚ for example‚ if two substances will mix or not. We can now do this by knowing that polar substances only mix with other polar substances‚ while non-polar substances only mix with other non-polar substances. This concept can be used to predict if a substance will be soluble in a specific solvent. The concepts of solubility and conductivity of a
Premium Chemical polarity Solubility Water
Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
Premium Enzyme PH Chemical reaction
Enzyme Kinetics Marcos‚ Nelissa S. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ABSTRACT The rationale of the experiment is basically founded in the concept of reaction rates as affected by enzyme‚ and how the enzyme works is competed by a competitive inhibitor‚ thereby impeding the forward reaction. In this experiment‚ o-diphenol oxidase‚ an enzyme that causes the browning in fruits‚ was extracted from banana and reaction rate of this was established
Premium Enzyme inhibitor Chemical kinetics Chemical reaction
Conclusion Shagun Mistry According to the experiment‚ the average normal heart rate of Daphnia is 20 heart beats per 12.94 seconds. There were six total trials done for Normal Heart Rate with 2 Daphnia (3 Trials each). The aim of this experiment was to measure the effect of neurotransmitters on the heart rate of Daphnia. When the adrenaline was added
Premium Heart rate Normal distribution Speed
Purpose: The purpose of this lab is to investigate the reliability of the activity series of metals by testing in solution‚ confirming if the activity series was correct with its predictions. Hypothesis: It is predicted that the metals in solid form will displace metals in solution if located higher over the other within the activity series chart. Materials: 10 test tubes Test tube holder Metals in Solid Form 1. Aluminum 2. Iron 3. Zinc 4. Magnesium 5. Zinc 6. Aluminum 7. Copper
Premium Metal Aluminium Copper
plant wide rate to allocate manufacturing overhead to jobs. The rate is computed by dividing expected manufacturing overhead by the expected denominator activity level. Expected manufacturing cost was $6‚000‚000 and the expected denominator activity level was 15‚000. The actual manufacturing overhead was $5‚600‚000 and the actual denominator activity level was 14‚000. Which of the following is the manufacturing overhead result for this period? (2 marks) a. $200‚000 over-applied b. $0 c. $400‚000 under-applied
Premium Economics Costs Cost
Digestion and Enzymes – APP. Hypothesis: The enzyme‚ Amylase which is used to break down carbohydrates will work the best when heated at 40°C. Also‚ as the temperature increases the reaction rate of amylase increases too. However‚ the reaction rate of amylase will start decreasing when the temperature reaches the enzyme’s optimal temperature. Many enzymes are specific for a certain substrate. For example‚ lipase is a specific enzyme for fat substrates and protease‚ a specific enzyme for protein
Free Enzyme
Action of enzymes as catalysts in biochemical processes * Enzymes acts as catalyst and increase the rate of all the chemical reactions. * Enzymes are also described by two properties like all other catalysts. It composed of two main functions. * The first function is that‚ they increase the rate of chemical reactions by without consumed themselves or undergo any change or alteration in the reaction. . ( Zemitec et‚al 2008). * The second function is‚ they increase reaction rates
Premium Glycolysis Adenosine triphosphate Cellular respiration
Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
Premium DNA Molecular biology Protein