Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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Buffers‚ and pH‚ and Diffusion oh my The pH of a solution is the measure of the concentration of charged Hydrogen ions in that given solution. A solution with a pH lower than seven is considered to be acidic. A solution with a higher pH is a base. It is very important for organisms to maintain a stable pH. Biological molecules such as proteins function only at a certain pH level and any changes in pH can result in them not functioning properly. To maintain these constant pH levels‚ buffer solutions
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------------------------------------------------- ------------------------------------------------- EXPERIMENT NO. 4 ------------------------------------------------- COLORIMETRIC DETERMINATION OF pH ------------------------------------------------- Abstract This experiment examined the result of the various color indicators combined with different buffer solution‚ thereby testing the precision and accuracy of determining the pH value colorimetrically. Colorimetric method is a simple and practical procedure with
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Varibles that affect Enzyme Catalysis Reaction Rates Introduction Molecules are constantly moving in our bodies and in nature. When molecules move fast enough they collide into one another‚ allowing chemical reactions to occur. Factors such as temperature and concentrations can either help increase or decrease these reactions. (Jubenville.) Enzymes are known as catalyst because they are able to speed up reaction rates without being destroyed or altered. They are able to encourage chemical reactions
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Enzymes are proteins or nucleic acids that catalyze reactions. They are able to speed up reactions by reducing the activation energy of a reaction. Each kind of enzyme has a specific shape that matches its substrate so it can bind to its active site. Enzymes convert their substrates into a product. Enzyme activity are affected by factors such as temperature‚ pH‚ and time. If an enzyme is exposed to extreme heat‚ it will become denatured‚ that is‚ to become deformed and lose its original shape which
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place where the experiment took place is the same Materials List: liver solution hydrogen peroxide 25% diluted hydrogen peroxide 50% diluted hydrogen peroxide 100% diluted distilled water (2) graduated cylinders 10 mL (4) 250 Beaker (1) stopwatch (1) 250 mL flask (1) tubing (1) Shallow pan for water bath (1) glass dropper tube (1) stopper (1) glass tube with opening at the bottom Procedures: 1. Fill a shallow pan 3/4th full of water and place the glass tube into the water and make sure that
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specifically with Medical Surgical students in clinical‚ lab and classroom settings. In her spare time Dixie enjoys spending time with her husband‚ three children‚ one grandson and her pets. She loves spending time outdoors and especially loves water-related activities like jet skiing and swimming. Congrats to Gale Winger for being elected to the student governance committee! Her advice to students is “School can get busy and crazy hectic at times‚ however‚ you must always remember to take a
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of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans Research Question: What is the effect of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans? Independent Variable: The different pH levels in the water would be the independent variable in this lab. Three different pH levels were tested‚ 4‚ 7 and 10‚ both 4 and 10 were chosen because they are 3 pH levels away from 7 which would be the regular pH level of water. We measured the different
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Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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Enzyme Catalysis Lab Problem: Before the lab‚ one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature‚ pH‚ enzyme concentration‚ and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase
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