Enzyme Catalysis Lab
Enzyme Catalysis Lab
Problem: Before the lab, one should understand:
• The general functions and activities of enzymes;
• The relationship between the structure and function of enzymes
• The concept of initial reaction rates of enzymes;
• How the concept of free energy relates to enzyme activity;
• That change in temperature, pH, enzyme concentration, and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and
• Catalyst, catalysis, and catalase
After the lab, one should be able to:
• Measure the effects of changes in temperature, pH, enzyme concentration, and substrate concentration on reaction rates of an enzyme-catalyzed reaction in a controlled experiment; and
• Explain how environmental factors affect the rate of enzyme-catalyzed reactions.
Hypothesis: When one mixes catalase and hydrogen peroxide with sulfuric acid for a short period of time, …show more content…
This was the index of the initial concentration of H2O2 in solution.
To establish a baseline, start by putting 10 mL of 1.5% H2O2 into a clean glass beaker. Add 1 mL of H2O (instead of enzyme solution). Add 10 mL of H2 SO4 (1.0 M). Mix well. Remove a 5-mL sample. Place this 5-mL sample into another beaker and assay for the amount of H2O2 as follows. Use a syringe to add KMnO4 , a drop at a time, to the solution until a persistent pink or brown color is obtained. Remember to gently swirl in the solution after adding each drop. Check to be sure that you understand the calibrations on the syringe. Record your reading.
Proceed now to Exercise 2D: An Enzyme-Catalyzed Rate of H2O2 Decomposition. In this experiment, we determined the rate at which a 1.5% H2O2 solution decomposes when catalyzed by the purified catalase extract. To do this, we determined how much H2O2 had been consumed after 10, 30, 60, 90, 120, and 180
Problem: Before the lab, one should understand:
• The general functions and activities of enzymes;
• The relationship between the structure and function of enzymes
• The concept of initial reaction rates of enzymes;
• How the concept of free energy relates to enzyme activity;
• That change in temperature, pH, enzyme concentration, and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and
• Catalyst, catalysis, and catalase
After the lab, one should be able to:
• Measure the effects of changes in temperature, pH, enzyme concentration, and substrate concentration on reaction rates of an enzyme-catalyzed reaction in a controlled experiment; and
• Explain how environmental factors affect the rate of enzyme-catalyzed reactions.
Hypothesis: When one mixes catalase and hydrogen peroxide with sulfuric acid for a short period of time, …show more content…
This was the index of the initial concentration of H2O2 in solution.
To establish a baseline, start by putting 10 mL of 1.5% H2O2 into a clean glass beaker. Add 1 mL of H2O (instead of enzyme solution). Add 10 mL of H2 SO4 (1.0 M). Mix well. Remove a 5-mL sample. Place this 5-mL sample into another beaker and assay for the amount of H2O2 as follows. Use a syringe to add KMnO4 , a drop at a time, to the solution until a persistent pink or brown color is obtained. Remember to gently swirl in the solution after adding each drop. Check to be sure that you understand the calibrations on the syringe. Record your reading.
Proceed now to Exercise 2D: An Enzyme-Catalyzed Rate of H2O2 Decomposition. In this experiment, we determined the rate at which a 1.5% H2O2 solution decomposes when catalyzed by the purified catalase extract. To do this, we determined how much H2O2 had been consumed after 10, 30, 60, 90, 120, and 180